雷蒙德氏棉HSP70基因家族的进化分析及其同源基因在陆地棉中的表达分析

doi:10.3724/SP.J.1005.2014.0921

遗传 ›› 2014, Vol. 36 ›› Issue (9): 921-933.doi: 10.3724/SP.J.1005.2014.0921

雷蒙德氏棉HSP70基因家族的进化分析及其同源基因在陆地棉中的表达分析

张毓婷^{1, 2}, 王敏华^{1, 2}, 陈家栋², 戎均康^{2, 3}, 丁明全^{2, 3}

1. 浙江农林大学,亚热带森林培育国家重点实验室培育基地,临安 311300; 2. 浙江农林大学农业与食品科学学院,浙江省农产品品质改良技术研究重点实验室,临安 311300; 3. 中国农业科学院棉花研究所,棉花生物学国家重点实验室,安阳 45500

收稿日期:2014-03-11 出版日期:2014-09-20 发布日期:2014-09-20
通讯作者: 丁明全,讲师,研究方向：棉花遗传与分子育种。E-mail：mqding@bjfu.edu.cn E-mail:zhangyutingly@126.com
作者简介:张毓婷,在读硕士研究生,专业方向：棉花遗传与分子育种。
基金资助:
国家高技术研究发展计划项目(863计划)(编号：2011AA100202),国家自然科学基金项目(编号： 31200909),浙江省自然科学基金项目(编号： LQ12C06002)和棉花生物学国家重点实验室开放课题(编号：CB2013A02,CB2014B04)资助

Genome-wide analysis of HSP70 superfamily in Gossypium raimondii and the expression of orthologs in Gossypium hirsutum

Yuting Zhang^{1, 2}, Minhua Wang^{1, 2}, Jiadong Chen², Junkang Rong^{2, 3}, Mingquan Ding^{2, 3}

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin’an 311300, China; 2. The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang Agricultural and Forestry University, Lin’an 311300, China; 3.Institute of Cotton Research, Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Anyang 455000, China

Received:2014-03-11 Online:2014-09-20 Published:2014-09-20

摘要/Abstract

摘要： 热激蛋白70家族(HSP70)是一类在植物中高度保守的分子伴侣蛋白,在细胞中协助蛋白质正确折叠。文章利用隐马可链夫模型(HMM)在雷蒙德氏棉(Gossypium raimondii L.)全基因组范围内进行HSP70基因家族成员进化分析,共得到30个HSP70家族成员。利用生物信息学对雷蒙德氏棉HSP70基因的结构、染色体分布、基因倍增模式以及系统进化进行分析,结果表明,HSP70基因家族根据亚细胞定位结果可分为不同的基因亚家族,各亚家族中HSP70基因具有相对保守的基因结构;染色体片段重复和串联重复是雷蒙德氏棉HSP70基因家族扩增的主要方式。通过对不同物种的HSP70基因家族进行系统进化分析可知,HSP70亚组的分化发生在单细胞植物形成前,且细胞质型HSP70成员大量扩增。比较陆地棉棉纤维发育不同时期的深度测序表达谱,发现HSP70基因可能参与棉纤维的生长发育。本研究结果有助于了解棉属植物HSP70基因家族的功能,以期为深入研究棉纤维发育过程中的分子调控机理提供基础。

关键词: 棉纤维, HSP70家族, 深度测序, 基因结构, 系统进化分析

Abstract: Heat shock 70 (HSP70) proteins are highly conserved molecular chaperones widely existed in the plant kingdom which are involved in cellular protein folding process. In this study, comprehensive evolutionary analyses of the Gossypium raimondii HSP70 gene family members are conducted and 30 HSP70 genes are identified. The gene structure, chromosome distribution, gene duplication and phylogenic evolution of this family are further analyzed. The results reveal that HSP70 family genes can be clustered into several major subgroups based on their sub-cellular locations, and the gene structures are relatively conserved in each subgroup. Both tandem duplications and chromosome segmental duplications are found to contribute to the expansion of HSP70 gene family. Evolutionary analysis of HSP70s in diverse species reveals that the differentiation of HSP70 subgroups occurred before the multi-cell and single-cell differentiation, with the cytoplasmic HSP70s multiple amplified. The expression pattern of HSP70 genes under series of fiber development stages indicates that many HSP70 genes may participate in fiber development processes including fiber initiation and elongation. This study provides the complete profiles of cotton HSP70 family genes for future study on their functions related to the molecular mechanisms of fiber development.

Key words: cotton fiber, HSP70 family, deep sequencing, gene structure, phylogenic analysis

张毓婷, 王敏华, 陈家栋, 戎均康, 丁明全. 雷蒙德氏棉HSP70基因家族的进化分析及其同源基因在陆地棉中的表达分析[J]. 遗传, 2014, 36(9): 921-933.

Yuting Zhang, Minhua Wang, Jiadong Chen, Junkang Rong, Mingquan Ding. Genome-wide analysis of HSP70 superfamily in Gossypium raimondii and the expression of orthologs in Gossypium hirsutum[J]. HEREDITAS(Beijing), 2014, 36(9): 921-933.

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雷蒙德氏棉HSP70基因家族的进化分析及其同源基因在陆地棉中的表达分析

Genome-wide analysis of HSP70 superfamily in Gossypium raimondii and the expression of orthologs in Gossypium hirsutum

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