遗传 ›› 2008, Vol. 30 ›› Issue (9): 1217-1222.doi: 10.3724/SP.J.1005.2008.01217

• 遗传学教学 • 上一篇    下一篇

选育1个玉米果穗验证遗传学三规律

赵文耀1; 刘俊波2; 赵亮3

  

  1. 1. 沈阳大学生物与环境学院, 沈阳 110034;
    2. 中国教育部教学仪器研究所, 北京 100080;
    3. 农安春光种业有限责任公司, 长春 130200
  • 收稿日期:2007-10-27 修回日期:2008-05-22 出版日期:2008-09-10 发布日期:2008-09-10
  • 通讯作者: 赵亮

Development of one corn ear and verification the three laws of inheritance

ZHAO Wen-Yao1;LIU Jun-Bo2;ZHAO Liang3   

  1. 1. College of Biology & Environmental Science, Shenyang University, Shenyang 110034, China;
    2. Educational Equipments Research Institute, Ministry of Education, Beijing 100080, China;
    3. Nongan Chunguang Breeding Company, Ltd., Changchun 130200, China
  • Received:2007-10-27 Revised:2008-05-22 Online:2008-09-10 Published:2008-09-10
  • Contact: ZHAO Liang

摘要:

为了选育新的实验材料, 予以1个F2代玉米果穗上不同籽粒性状的分离比, 验证遗传学三规律, 依据遗传学三规律研究内容, 选择玉米第4对染色体上甜质胚乳基因Sn1sn1、第9对染色体上粒色基因C1c1和糯质胚乳基因Wxwx, 这3对基因所调控的相对性状为原始试材。经连续多代自交与测交、正交与反交, 严格选择, 终于选育出基因型纯合的亲本, 组配成杂交种。文章以“玉米基因交换值”和减数分裂所形成的配子类型、数量, 推导出理论值来衡量新的实验材料F2代同一个果穗上不同籽粒性状的分离比。结果显示: 自交穗呈现出3个 3︰1、2个9︰3︰3︰1、1个连锁交换, c2值均小于准许值。即准确、恰当验证遗传学三规律, 又将遗传学三规律融合在一起, 形成完整统一体, 选育出目前最新的实验材料。

关键词: 融合, 遗传学规律, 籽粒性状, 玉米果穗

Abstract:

A new corn was developed as a lab material to verify the three Laws of Inheritance on only one F2 ear with correct segregation ratios of kernel traits. In order to investigate the three Inheritance Laws simultaneously with one ear, the sweet endosperm (Sn1), kernel color (C1) and the glutinous endosperm (Wx) were chosen as the original phenotypes. Gene Sn1sn1 is located on chromosome four, genes C1c1 and Wxwx are located on chromosome nine. After several generations of continuous self-pollination and test crossing, direct crossing and back crossing, with strict selections, the homozygous parents were ob-tained. With different combinations of homozygous parents, different hybrids were developed. The segregation ratios of each phenotype on the same F2 ear are compared to the theoretical segregation ratios according to the corn gene exchange and gam-ete types and quantities produced through meiosis. The results showed three ratios of 3︰1, two ratios of 9︰3︰3︰1, and one ratio of linkage and exchange, with all the chi-square values smaller than the permitted ones. This means that only one ear of this new hybrid corn can be used as a new teaching aid to examine and verify the three Laws of Inheritance.