遗传 ›› 2000, Vol. 22 ›› Issue (2): 78-80.
王为未1;张丽珊1;黄鹰1;周晓雷1;洪泽辉1;龚嫦虹1;黄志纯1;2 WANG Wei-wei1;ZHANG Li-shan1;HUANG Ying1;ZHOU Xiao-lei1;HONG Ze-hui1;GONG Chang-hong1;HUANG Zhi-chun1;2
摘要: 应用PCR、PCR-SSCP和DNA序列分析等分子生物学技术,对一个有明确氨基糖苷类抗生素应用史的母系遗传耳聋家系共8人(包括聋人和听力正常者) 的线粒体DNA进行研究,结果显示,家系中有4份样品存在线粒体DNA 12S rRNA 1 555位点A→G的突变。提示线粒体DNA点突变是导致该家系致聋的主要因素之一。
Abstract:Blood samples were obtained from a pedigree with aminoglycoside antibiotic induced deafness.DNA was extracted from the isolated leukocytes.The mitochondrial DNA fragments were detected by PCR-SSCP and DNA sequencing.It was found that four individuals from the pedigree carried 1 555 A→G mutation.From our results,mitochondrial DNA mutation may be one of major factors in aminoglycoside antibiotic induced deafness.