遗传 ›› 2004, Vol. 26 ›› Issue (3): 0-329.
• 论文 • 下一篇
王宏林1; 喻德跃1; 王永军2; 陈受宜2; 盖钧镒1 WANG Hong-Lin1; YU De-Yue1; WANG Yong-Jun2; CHEN Shou-Yi2; GAI Jun-Yi1
摘要:
应用构建的NJRIKY(科丰1号×1138-2)大豆重组自交家系群体,对大豆根重QTL进行8次重复的随机区组分析;以该群体所构成的遗传连锁图谱为基础,采用复合区间作图法(Cartographer V. 1.21)检测到3个与根重有关的QTL位于连锁群N3-B1和N6-C2上。其中rw1在N3-B1的端距离是66.31cM,位于A520T~ACCCAGO5 区间,rw2和rw3分别在N6-C2的端距离是 169.91cM和179.71 cM,并与OPW13和ACGCATO6重叠。 LOD值分别是10.34、4.01和3.15,可以解释26.3%、9.2%和6.8% 的遗传变异。加性效应估计值分别为-0.514、-0.303和-0.260。 Abstract: The recombinant inbred line (RIL) population, NJRIKY with 184 families derived from a cross Kefeng No.1 × Nannong1138-2 was used in mapping QTLs of root weight of soybean. Based on the linkage map constructed by Wang, with the software Cartographer V. 1.21 of the composite interval mapping procedure, three QTLs of root weight were mapped on N3-B1 and N6-C2 linkage group. The left telomere distance of rw1 was 66.31cM on N3-B1 linkage group, and those of rw2 and rw3 were 169.91cM and 179.71 cM,respectively, on N6-C2 linkage group. The former was located in A520T~ACCCAGO5 and the latter were overlapped with OPW13 and ACGCATO6, respectively. Their LOD values were 10.34、4.01 and 3.15, respectively. The QTLs of the root weight explained 26.3%、9.2% and 6.8% of the total variation, and their additive effects were -0.514、-0.303 and -0.260, respectively.