遗传 ›› 2001, Vol. 23 ›› Issue (2): 119-122.

• 论文 • 上一篇    下一篇

用焦磷酸测序技术研究猪线粒体细胞色素B基因单倍型

蒋思文;U.Gustafsson2;E.Giuffra2;L.Andersson2;熊远著1 JIANG Si-wen1;U.Gustafsson2;E.Giuffra2;L.Andersson2;XIONG Yuan-zhu1   

  1. 1.华中农业大学畜牧兽医学院,农业部猪遗传育种重点实验室,武汉 430070; 2.瑞典农业大学动物遗传育种系,瑞典 1.College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070; 2.Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala BMC, S-751 24 Uppsala, Sweden
  • 收稿日期:1900-01-01 出版日期:2001-04-10 发布日期:2001-04-10

Porcine Mitochondrial Cytochrome B Gene Haplotypes Detection by Pyrosequencing

  • Received:1900-01-01 Online:2001-04-10 Published:2001-04-10

摘要: 选择43头大白猪,79头长白猪,66头皮特兰猪和60头清平猪作试验材料,采用焦磷酸测序技术分析猪线粒体细胞色素b(CytB)基因单倍型。研究结果显示CytB基因可分为4种单倍型E1,E2,A1和A2。清平猪仅存在于A1单倍型(100%),大白猪和长白猪存在于E1(49.19%,79.25%)和A1(55.81%,20.25%)单倍型,皮特兰则存在于E1(57.58%)和A2(42.42%)单倍型。
Abstract:To detect porcine mitochondrial cytochrome b (CytB) gene haplotypes, Pyrosequencing, which is a novel DNA sequencing method, has been used to analyze SNPs selected Large White, Landrace, Pietrain and Qingping pigs. The pyrosequencing analysis of CytB gene displayed four distinct haplotypes E1, E2, A1 and A2 respectively. Qingping pigs are only present in haplotype A1, Large White and Landrace pigs are present in haplotype E1 and A1, and Pietrain pigs are present in haplotupe E1 and A2.

关键词: 线粒体DNA, 细胞色素b基因, 焦磷酸测序 Keywords, 单倍型, mitochondrial DNA