质膜组分磷脂酰丝氨酸外翻的分子调控机制

doi:10.16288/j.yczz.14-207

遗传 ›› 2015, Vol. 37 ›› Issue (2): 140-147.doi: 10.16288/j.yczz.14-207

质膜组分磷脂酰丝氨酸外翻的分子调控机制

毕丹^1,2,徐扬^1,2,逄越^1,2,李庆伟^1,2

1. 辽宁师范大学生命科学学院,大连 116029;
2. 辽宁师范大学七鳃鳗研究中心,大连 116029

收稿日期:2014-06-25 出版日期:2015-02-20 发布日期:2015-01-19
通讯作者: 李庆伟,教授,博士生导师,研究方向：细胞生物学。E-mail: liqw@263.net;逄越,副教授,硕士生导师,研究方向：细胞生物学。E-mail: pangyue01@163.com
作者简介:毕丹,硕士研究生,专业方向：细胞生物学。E-mail: 543272866@qq.com
基金资助:
国家重大基础研究发展规划(973计划)项目(编号：2013CB835304),全国海洋公益项目(编号：201305016),国家自然科学基金项目(编号：31170353,31202020)和大连市科技计划项目(编号：2013E11SF056)资助

Molecular regulations of phosphatidylserine eversion in plasma membrane

Dan Bi^1,2,Yang Xu^1,2,Yue Pang^1,2,Qingwei Li^1,2

1. College of Life Science, Liaoning Normal University, Dalian 116029, China;
2. Lamprey Research Center, Liaoning Normal University, Dalian 116029, China

Received:2014-06-25 Online:2015-02-20 Published:2015-01-19

摘要/Abstract

摘要： 磷脂酰丝氨酸(Phosphatidylserine, PS)是细胞质膜重要的磷脂成分之一,具有重要的生物学功能。在细胞凋亡及一些特殊的病理条件下,细胞内ATP供能不足,胞浆Ca²⁺浓度升高,引起PS发生外翻。PS外翻在不同类型细胞中具有不同的生物学功能,且外翻的程度与疾病发展程度密切相关,可作为癌症等多种疾病治疗的靶标。文章综述了细胞质膜中磷脂酰丝氨酸的重要生物学功能和意义、磷脂酰丝氨酸外翻的分子机制及在临床医学方面的应用,以期对未来的功能和临床应用研究提供参考。

关键词: 磷脂酰丝氨酸, 靶标分子, 细胞凋亡, 磷脂酰丝氨酸外翻

Abstract: Phosphatidylserine (PS), one important phospholipid of cell membranes, has crucial biological functions. Under special pathological circumstances such as cell apoptosis, cells cannot generate enough ATP for energy and the concentration of cytoplasmic Ca²⁺ increases, resulting in PS eversion. PS eversion has different biological functions in different cell types. In addition, the degree of eversion is closely associated with the progress of diseases. Therefore, it can be therapeutic targets of cancer and many other diseases. In this review, we summarize the fundamental biological functions of PS, molecular mechanisms of PS eversion, as well as its potential in translational medicine.

Key words: phosphatidylserine, target molecules, apoptosis, phosphatidylserine eversion

毕丹,徐扬,逄越,李庆伟. 质膜组分磷脂酰丝氨酸外翻的分子调控机制[J]. 遗传, 2015, 37(2): 140-147.

Dan Bi,Yang Xu,Yue Pang,Qingwei Li. Molecular regulations of phosphatidylserine eversion in plasma membrane[J]. HEREDITAS(Beijing), 2015, 37(2): 140-147.

参考文献

[1] 王镜岩, 朱圣庚, 徐长法. 生物化学. 北京: 高等教育出版社, 2002.
[2] Folch J, Lees M, Sloane-Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem , 1957, 226(1): 497-509.
[3] Baer E, Maurukas J. Phosphatidyl serine . J Biol Chem , 1955, 212(1): 25-38.
[4] Chu C, Huang X, Chen CT, Zhao Y, Luo JJ, Gray BD, Pak KY, Dun NJ. In vivo imaging of brain infarct with the novel fluorescent probe PSVue 794 in a rat middle cerebral artery occlusion-reperfusion model. Mol Imaging , 2013, 12(1): 8-16.
[5] Lin QQ, London E. Preparation of artificial plasma membrane mimicking vesicles with lipid asymmetry. PLoS One , 2014, 9(1): e87903.
[6] Haberkorn U, Markert A, Mier W, Askoxylakis V, Altmann A. Molecular imaging of tumor metabolism and apoptosis. Oncogene , 2011, 30(40): 4141-4151.
[7] Seigneuret M, Devaux PF. ATP-dependent asymmetric distribution of spin-labeled phospholipids in the erythrocyte membrane: relation to shape changes. Proc Natl Acad Sci USA , 1984, 81(12): 3751-3755.
[8] Haest CWM. Distribution and movement of membrane lipids. In: Bernhardt I, Ellory C. Red Cell Membrane Transport in Health and Disease. Berlin Heidelberg: Springer, 2003: 1-25.
[9] Ran S, Downes A, Thorpe PE. Increased exposure of anionic phospholipids on the surface of tumor blood vessels. Cancer Res , 2002, 62(21): 6132-6140.
[10] Kroy DC, Ciuffreda D, Cooperrider J H, Kroy DC, Ciuffreda D, Cooperrider JH, Tomlinson M, Hauck GD, Aneja J, Berger C, Wolski D, Carrington M, Wherry EJ, Chung RT, Tanabe KK, Elias N, Freeman GJ, de Kruyff RH, Misdraji J, Kim AY, Lauer GM. Liver environment and HCV replication affect human T-cell phenotype and expression of inhibitory receptors. Gastroenterology , 2014, 146(2): 550-561.
[11] Ravichandran KS. Beginnings of a good apoptotic meal: the find-me and eat-me signaling pathways. Immunity , 2011, 35(4): 445-455.
[12] Caberoy NB, Alvarado G, Bigcas JL, Li W. Galectin-3 is a new MerTK-specific eat-me signal. J Cell Physiol , 2012, 227(2): 401-407.
[13] Dong HP, Holth A, Kleinberg L, Ruud MG, Elstrand MB, Tropé CG, Davidson B, Risberg B. Evaluation of cell surface expression of phosphatidylserine in ovarian carcinoma effusions using the annexin-V/7-AAD assay clinical relevance and comparison with other apoptosis parameters. Am J Clin Pathol , 2009, 132(5): 756-762.
[14] Jilani K, Enkel S, Bissinger R, Almilaji A, Abed M, Lang F. Fluoxetine induced suicidal erythrocyte death. Toxins (Basel) , 2013, 5(7): 1230-1243.
[15] Bhavsar SK, Bobbala D, Xuan NT, Föller M, Lang F. Stimulation of suicidal erythrocyte death by α-lipoic acid. Cell Physiol Biochem , 2010, 26(6): 859-868.
[16] Jilani K, Lang F. Carmustine-induced phosphatidylserine translocation in the erythrocyte membrane. Toxins (Basel) , 2013, 5(4): 703-716.
[17] Weiss E, Rees DC, Gibson JS. Role of calcium in phosphatidylserine externalisation in red blood cells from sickle cell patients. Anemia , 2010, 7(4): 1-8.
[18] 刘述川, 周晋, 史家岚, 苏雁华. 正常血细胞表面磷脂酰丝氨酸的表达及其促凝功能. 中华血液学杂志, 2011, 32(12): 858-862.
[19] Laliberte JP, Moss B. Appraising the apoptotic mimicry model and the role of phospholipids for poxvirus entry. Proc Natl Acad Sci USA , 2009, 106(41): 17517-17521.
[20] Farias LH, Rodrigues A P, Silveira FT, Seabra SH, DaMatta RA, Saraiva EM, Silva EO. Phosphatidylserine exposure and surface sugars in two Leishmania (Viannia) braziliensis strains involved in cutaneous and mucocutaneous leishmaniasis. J Infect Dis , 2013, 207(3): 537-543.
[21] Rochael NC, Lima LG, Oliveira SM, Barcinski MA, Saraiva EM, Monteiro RQ, Pinto-da-Silva LH. Leishmania amazonensis exhibits phosphatidylserine-dependent procoagulant activity, a process that is counteracted by sandfly saliva. Mem Inst Oswaldo Cruz , 2013, 108(6): 679-685.
[22] Riedl S, Rinner B, Asslaber M, Schaider H, Walzer S, Novak A, Lohner K, Zweytick D. In search of a novel target—phosp

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

质膜组分磷脂酰丝氨酸外翻的分子调控机制

Molecular regulations of phosphatidylserine eversion in plasma membrane

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics

[1]	王翠玲, 刘信燚, 王亚会, 张争, 王治东, 周钢桥. MCM2通过抑制p53信号通路促进胆管癌细胞的增殖、迁移和侵袭[J]. 遗传, 2022, 44(3): 230-244.
[2]	秦中勇, 石晓, 曹平平, 褚鹰, 管蔚, 杨楠, 程禾, 孙玉洁. 细胞凋亡反应中NOXA基因启动子发挥增强子功能调节BCL2基因表达[J]. 遗传, 2020, 42(11): 1110-1121.
[3]	余同露,蔡栋梁,朱根凤,叶晓娟,闵太善,陈红岩,卢大儒,陈浩明. CSN4基因干扰对乳腺癌MDA-MB-231细胞增殖和凋亡的影响[J]. 遗传, 2019, 41(4): 318-326.
[4]	赵建元,丁寄葳,米泽云,周金明,魏涛,岑山. HIV-1初始传播病毒Vpr基因遗传变异对诱导G₂期阻滞及细胞凋亡的影响[J]. 遗传, 2015, 37(5): 480-486.
[5]	张蕾, 隋御, 王婷, 李利坚, 李元杰, 金彩霞, 徐方. hMMS2基因对结肠癌细胞耐药逆转的影响[J]. 遗传, 2014, 36(4): 346-353.
[6]	王师尧，金巍娜，吴丹. 青少年型神经元蜡样脂褐质沉积病(JNCL)的发病机制[J]. 遗传, 2009, 31(8): 779-784.
[7]	马向东，马兴，吴小明，陈必良，王德堂 . 胚胎神经管缺陷大鼠模型差异基因的表达[J]. 遗传, 2009, 31(3): 280-284.
[8]	郭芬，刘兆宇，李月琴，李弘剑，周天鸿. Prosaposin对细胞增殖和凋亡的调控及其分子机制[J]. 遗传, 2009, 31(12): 1226-1232.
[9]	付浩，赵丹懿，孙秀菊，滑君，阎杨，邱广蓉，尚超，富伟能，孙开来. B-RAF基因特异的siRNA干扰对胃癌BGC823细胞的影响[J]. 遗传, 2007, 29(5): 537-537―540.
[10]	王琳，梁旭方，廖婉琴，周天鸿. 斑马鱼胚胎发育中细胞凋亡的研究进展[J]. 遗传, 2006, 28(8): 1009-1014.
[11]	王振兴，王璞，姚玲，曾智东，徐汉福，段军，王根洪，夏庆友. 家蚕细胞凋亡相关基因BmICAD的克隆、序列和功能分析及其在精巢中特异表达的初步研究[J]. 遗传, 2006, 28(7): 838-844.
[12]	李艳凤，张强，朱大海. 泛素介导的蛋白质降解与肿瘤发生[J]. 遗传, 2006, 28(12): 1591-1591~1596.