遗传 ›› 2003, Vol. 25 ›› Issue (2): 181-184.

• 论文 • 上一篇    下一篇

转基因番木瓜的抗病性及分子鉴定

叶长明1;魏祥东1;陈东红1;蓝崇钰1;朱利民2 YE Chang-Ming1;WEI Xiang-Dong1;CHEN Dong-Hong1;LAN Chong-Yu1;ZHU Li-Min2   

  1. 1.中山大学基因工程教育部重点实验室 生物防治国家重点实验室,广州 510275; 2.香港中文大学生物系,香港沙田 Laboratory of Biological Control,Zhongshan University,Guangzhou 510275,China; 2.Department of Biology,The Chinese University of Hong Kang
  • 收稿日期:1900-01-01 出版日期:2003-04-10 发布日期:2003-04-10

Analyses of Virus Resistance And Transgenes for Transgenic Papaya

  • Received:1900-01-01 Online:2003-04-10 Published:2003-04-10

摘要: 对T1代转番木瓜环斑病毒(PRV)复制酶(RP)突变体基因的两个番木瓜株系,进行了抗病性和分子生物学分析。结果表明,转基因番木瓜对PRV抗性达到高抗或免疫,目的基因RP遗传至转基因后代并在RNA水平表达,PCR可检测到CaMV35S启动子序列、标记基因NPTII。
Abstract:Virus resistance in field and molecular biological characterizations of the transgenes were analyzed for two lines of T1 generation of transgenic papaya with the replicase mutant gene from papaya ringspot virus (PRV).The transgenic plants showed highly resistant or immune against PRV.Results indicated that the transgenes inherited to and expressed at RNA level in the progenies.

关键词: 转基因番木瓜, 抗病性, 检测
Key words,
virus resistance, transgenic papaya