遗传 ›› 2003, Vol. 25 ›› Issue (2): 181-184.
叶长明1;魏祥东1;陈东红1;蓝崇钰1;朱利民2 YE Chang-Ming1;WEI Xiang-Dong1;CHEN Dong-Hong1;LAN Chong-Yu1;ZHU Li-Min2
摘要: 对T1代转番木瓜环斑病毒(PRV)复制酶(RP)突变体基因的两个番木瓜株系,进行了抗病性和分子生物学分析。结果表明,转基因番木瓜对PRV抗性达到高抗或免疫,目的基因RP遗传至转基因后代并在RNA水平表达,PCR可检测到CaMV35S启动子序列、标记基因NPTII。
Abstract:Virus resistance in field and molecular biological characterizations of the transgenes were analyzed for two lines of T1 generation of transgenic papaya with the replicase mutant gene from papaya ringspot virus (PRV).The transgenic plants showed highly resistant or immune against PRV.Results indicated that the transgenes inherited to and expressed at RNA level in the progenies.