遗传 ›› 2003, Vol. 25 ›› Issue (2): 195-197.

• 论文 • 上一篇    下一篇

ERIC-PCR技术在李斯特氏菌种、菌株鉴定中的应用

金莉莉;王秋雨;侯潇 JIN Li-Li;WANG Qiu-Yu;HOU Xiao   

  1. 辽宁大学生命科学系,沈阳 110036 Biological Science Department of Liaoning University,Shenyang 110036,China
  • 收稿日期:1900-01-01 出版日期:2003-04-10 发布日期:2003-04-10

ERIC-PCR on Identification of Listeria Species and Strain

  • Received:1900-01-01 Online:2003-04-10 Published:2003-04-10

摘要: 应用肠杆菌基因间重复一致序列聚合酶链反应技术(ERIC-PCR)对李斯特氏菌基因组DNA进行分析,结果显示,李斯特氏菌种间DNA指纹图谱带型差异较大;单核细胞增生性李斯特氏菌株间及相同血清型不同来源的菌株,其DNA指纹图谱带型也有明显差异。在单核细胞增生性李斯特氏菌各株的DNA指纹图谱中发现1600bp的种专一性扩增带。结果表明,ERIC-PCR技术可用于李斯特氏菌种、菌株的鉴定及进一步分型研究。
Abstract:Enterobacteia repetitive intergenic consensus sequences-based PCR(ERIC-PCR) was used to generate DNA fingerprints for Listeria spp.We got the specific profiles with ERIC-PCR technique that enables to identify Listeria species and the L.monocytogenes strains of different sterotype,and the same sterotype of L.monocytogenes from different sources also could be identified.Moreover,the species-spcific 1600bp DNA fragment was obtained from the fingerprint of L.monocytogenes.The study indicates that ERIC-PCR technique can be used in the identification of Listeria species and strains and its further typing,which is simple and quickly.

关键词: DNA指纹图谱
Key words,
enterobacteia repetitive intergenic consensus sequences, 李斯特氏菌, PCR, 肠杆菌基因间重复一致序列