植物基因组编辑检测方法

doi:10.16288/j.yczz.18-079

遗传 ›› 2018, Vol. 40 ›› Issue (12): 1075-1091.doi: 10.16288/j.yczz.18-079

植物基因组编辑检测方法

刘春霞,耿立召,许建平()

先正达北京创新中心,北京 102206

收稿日期:2018-03-29 修回日期:2018-08-18 出版日期:2018-12-20 发布日期:2018-09-06
通讯作者: 许建平 E-mail:jianping.xu@syngenta.com
作者简介:刘春霞,博士,专业方向：作物遗传育种,植物基因组编辑。E-mail: chunxia. liu@syngenta.com

Detection methods of genome editing in plants

Chunxia Liu,Lizhao Geng,Jianping Xu()

Syngenta Beijing Innovation Center, Beijing 102206, China

Received:2018-03-29 Revised:2018-08-18 Online:2018-12-20 Published:2018-09-06
Contact: Xu Jianping E-mail:jianping.xu@syngenta.com

摘要/Abstract

摘要：

以CRISPR/Cas9技术为代表的基因组编辑在生物领域的革命性应用使得生命科学研究迈入新篇章。该技术以其灵活性、易用性且扩展性强等优势,大大加快了基因工程研究,也加速了植物分子育种的步伐。但是,遗传转化过程中产生大量潜在的基因编辑植株,使得早期高通量快速筛选和检测目标编辑植株面临很大挑战。本文综述了近年来植物基因组编辑检测的各种方法,比较了其优缺点和适用范围;同时,还对近几年植物基因组编辑检测方法的发展趋势进行了深入分析和展望,以期对基因组编辑技术在植物中的应用提供参考。

关键词: 非同源末端连接, 同源重组, PCR/RE, 错配切割, Sanger测序法

Abstract:

The life science has entered a new chapter with the revolutionary implementation of the CRISPR/Cas9 genome editing technology in various living organisms. With the unique flexibility, feasibility and extendibility, the CRISPR/Cas9 technology greatly accelerates genetic engineering research, as well as plant molecular breeding. However, it has become a challenge to screen for and identify genome-edited plants at early stages in a rapid and high-throughput fashion, due to the massive number of plants produced from transformation process. In this review, we summarize the molecular methods developed in recent years to identify genome-edited plants. We compare their advantages and disadvantages, and the scope of application. In addition, we provide insights of the development trend of detection methods for plant genome editing. This review will serve as a reference for future genome editing research in plants.

Key words: non-homologous end joining (NHEJ), homologous recombination (HR), PCR/RE, mismatch cleavage assay, Sanger sequencing

刘春霞, 耿立召, 许建平. 植物基因组编辑检测方法[J]. 遗传, 2018, 40(12): 1075-1091.

Chunxia Liu, Lizhao Geng, Jianping Xu. Detection methods of genome editing in plants[J]. Hereditas(Beijing), 2018, 40(12): 1075-1091.

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