小鼠不同泌乳期乳腺脂肪合成相关基因的mRNA表达

doi:10.3724/SP.J.1005.2012.00335

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HEREDITAS ›› 2012, Vol. 34 ›› Issue (3): 335-341.doi: 10.3724/SP.J.1005.2012.00335

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mRNA expression of lipogenic genes in mouse mammary gland in different lactation stages

HAN Li-Qiang¹, LI Hong-Ji¹, WANG Yue-Ying¹, WANG Lin-Feng¹, YANG Guo-Qing¹, WANG Yan-Ling², YANG Guo-Yu²

1. College of Animal Husbandry and Veterinary Science, Henan Agriculture University, Zhengzhou 450002, China 2. Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450002, China

Received:2011-05-19 Revised:2011-06-03 Online:2012-03-20 Published:2012-03-25

Abstract

Abstract: To investigate the expression pattern of lipogenic genes in mammary gland of mouse in different lactation stages, the relative mRNA abundance and expression of 20 genes involved in milk fat synthesis and secretion of lactating mammary tissues were assessed using real-time PCR. Results revealed the significant upregulation of mRNA expression for both high abundant genes (abundance > 5%), such as LPL, ACACA, SCD, XDH, BTN, and ADFP and moderate abundant genes (5% > abundance > 1%), such as CD36, FASN, AGPAT6, and DGAT in lactation stages compared to pregnancy (P < 0.05). The mRNA expression of lipogenic enzyme genes for ACACA, SCD, FASN, AGPAT6, and DGAT exhibited lower expression lever in early (6 d) and late (18 d) lactation stages but higher expression level at middle stage (12 d), demonstrating a low-high-low pattern. Besides, the mRNA levels of some gene regulators were also measured. The mRNA expression of SREBF gene increased gradually along with the lactation, which showed a 10-fold elevation at middle stage (12 d). The expression pattern of SREBF gene was the same as lipogenic enzyme genes, suggesting that SREBF may play an important role in the regulation of lipogenic enzyme genes in the lactating mammary gland.

Key words: lipid synthesis, mammary tissue, gene expression, SREBF

HAN Li-Qiang, LI Hong-Ji, WANG Yue-Ying, WANG Lin-Feng, YANG Guo-Qing, WANG Yan-Ling, YANG Guo-Yu. mRNA expression of lipogenic genes in mouse mammary gland in different lactation stages[J]. HEREDITAS, 2012, 34(3): 335-341.

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mRNA expression of lipogenic genes in mouse mammary gland in different lactation stages

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