CRISPR/Cas9系统在昆虫中的应用

doi:10.16288/j.yczz.17-263

[an error occurred while processing this directive]

Hereditas(Beijing) ›› 2018, Vol. 40 ›› Issue (4): 266-278.doi: 10.16288/j.yczz.17-263

Previous Articles Next Articles

Applications of the CRISPR/Cas9 system in insects

Xiaoling Tong^1,²,Chunyan Fang^1,²,Tingting Gai^1,²,Jin Shi^1,²,Cheng Lu^1,²,Fangyin Dai^1,²()

^1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China
^2. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, College of Biotechnology, Southwest University, Chongqing 400715, China

Received:2017-10-16 Revised:2018-01-21 Online:2018-04-20 Published:2018-01-24
Contact: Dai Fangyin E-mail:fydai@swu.edu.cn
Supported by:
Supported by the The National High Technology Research and Development Program of China (863 Program)(2013AA102507);the National Natural Science Foundation of China(31472153);the National Natural Science Foundation of China(31372379);the Technical System Special of Modern Agricultural industry of China(CARS-18)

Abstract

Abstract:

The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated) system guides Cas9 to specific genomic locations by a short RNA search string. This technology enables the systematic interrogation of mammalian genome editing, repairing damaged genes, silencing harmful genes and improving quality traits. In recent years, with the introduction of the CRISPR/Cas9 system for easy, fast and efficient genetic modification, it has been possible to conduct meaningful functional studies in a broad array of insect species, such as Drosophila, Bombyx mori, Aedes aegypti and butterflies et al. In this review, we summarize the application of CRISPR/Cas9 in different insect species, discuss methods for its promotion, and consider its application for future insect studies.

Key words: CRISPR/Cas9, genome editing, insects, application

Xiaoling Tong,Chunyan Fang,Tingting Gai,Jin Shi,Cheng Lu,Fangyin Dai. Applications of the CRISPR/Cas9 system in insects[J]. Hereditas(Beijing), 2018, 40(4): 266-278.

References

[1]	Kim YG, Cha J, Chandrasegaran S . Hybrid restriction enzymes: zinc finger fusion toFokⅠ cleavage domain. Proc Natl Acad Sci USA, 93(3):1156-1160.
[2]	Li T, Huang S, Zhao XF, Wright DA, Carpenter S, Spalding MH, Weeks DP, Bing Y . Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes. Nucleic Acids Res, 2011,39(14):6315-6325.
[3]	Moscou MJ, Bogdanove AJ . A simple cipher governs DNA recognition by TAL effectors . Science, 2009,326(5959):1501-1501.
[4]	Cong L, Ran FA, Cox D, Lin SL, Barretto R, Habib N, Hsu PD, Wu XB, Jiang WY, Marraffini LA, Zhang F . Multiplex genome engineering using CRISPR/Cas systems. Science, 2013,339(6121):819-823.
[5]	Jiang WY, Bikard D, Cox D, Zhang F, Marraffini LA . RNA-guided editing of bacterial genomes using CRISPR- Cas systems. Nat Biotechnol, 2013,31(3):233-239.
[6]	Garneau JE, Dupuis Mè, Villion M, Romero DA, Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S . The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature, 2010,468(7320):67-71.
[7]	Deltcheva E, Chylinski K, Sharma CM, Gonzales K, Chao Y, Pirzada ZA, Eckert MR, Vogel J, Charpentier E . CRISPR RNA maturation by trans-encoded small RNA and host factor RNaseⅢ. Nature, 2011,471(7340):602-607.
[8]	Jackson SP . Sensing and repairing DNA double-strand breaks. Carcinogenesis, 2002,23(5):687-696.
[9]	Mali P, Yang LH, Esvelt KM, Aach J, Guell M , DiCarlo JE, Norville JE, Church GM. RNA-guided human genome engineering via Cas9. Science, 2013,339(6121):823-826.
[10]	Jinek M, East A, Cheng A, Lin S, Ma EB, Doudna J . RNA-programmed genome editing in human cells. eLife, 2013,2:e00471.
[11]	Ren XJ, Sun J, Housden BE, Hu YH, Roesel C, Lin SL, Liu LP, Yang ZH, Mao DC, Sun LZ, Wu QJ, Ji JY, Xi JZ, Mohr SE, Xu J, Perrimon N, Ni JQ . Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9. Proc Natl Acad Sci USA, 2013,110(47):19012-19017.
[12]	Bassett A, Liu JL . CRISPR/Cas9 mediated genome engineering in Drosophila. Methods, 2014,69(2):128-136.
[13]	Yu ZS, Ren MD, Wang ZX, Zhang B, Rong YS, Jiao RJ, Gao GJ . Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila. Genetics, 2013,195(1):289-291.
[14]	Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J , O'Connor-Giles KM. Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics, 2013,194(4):1029-1035.

[1]	Xiaoping Lian, Guangfu Huang, Yujiao Zhang, Jing Zhang, Fengyi Hu, Shilai Zhang. The discovery and utilization of favorable genes in Oryza longistaminata [J]. Hereditas(Beijing), 2023, 45(9): 765-780.
[2]	Bingzheng Wang, Chao Zhang, Jiali Zhang, Jin Sun. Conditional editing of the Drosophila melanogaster genome using single transcripts expressing Cas9 and sgRNA [J]. Hereditas(Beijing), 2023, 45(7): 593-601.
[3]	Zhongsheng Wu, Yu Gao, Yongtao Du, Song Dang, Kangmin He. The protocol of tagging endogenous proteins with fluorescent tags using CRISPR-Cas9 genome editing [J]. Hereditas(Beijing), 2023, 45(2): 165-175.
[4]	Meizhen Liu, Liren Wang, Yongmei Li, Xueyun Ma, Honghui Han, Dali Li. Generation of genetically modified rat models via the CRISPR/Cas9 technology [J]. Hereditas(Beijing), 2023, 45(1): 78-87.
[5]	Xiaojun Zhang, Kun Xu, Juncen Shen, Lu Mu, Hongrun Qian, Jieyu Cui, Baoxia Ma, Zhilong Chen, Zhiying Zhang, Zehui Wei. A CRISPR/Cas9-Gal4BD donor adapting system for enhancing homology-directed repair [J]. Hereditas(Beijing), 2022, 44(8): 708-719.
[6]	Chong Zhang, Zixuan Wei, Min Wang, Yaosheng Chen, Zuyong He. Editing MC1R in human melanoma cells by CRISPR/Cas9 and functional analysis [J]. Hereditas(Beijing), 2022, 44(7): 581-590.
[7]	Ziwen Shi, Qing He, Zhuofan Zhao, Xiaowei Liu, Peng Zhang, Moju Cao. Exploration and utilization of maize male sterility resources [J]. Hereditas(Beijing), 2022, 44(2): 134-152.
[8]	Yao Liu, Xianhui Zhou, Shuhong Huang, Xiaolong Wang. Prime editing: a search and replace tool with versatile base changes [J]. Hereditas(Beijing), 2022, 44(11): 993-1008.
[9]	Yuting Han, Bowen Xu, Yutong Li, Xinyi Lu, Xizhi Dong, Yuhao Qiu, Qinyun Che, Ruibao Zhu, Li Zheng, Xiaochen Li, Xu Si, Jianquan Ni. The cutting edge of gene regulation approaches in model organism Drosophila [J]. Hereditas(Beijing), 2022, 44(1): 3-14.
[10]	Haitao Wang, Tingting Li, Xun Huang, Runlin Ma, Qiuyue Liu. Application of genetic modification technologies in molecular design breeding of sheep [J]. Hereditas(Beijing), 2021, 43(6): 580-600.
[11]	Guangwu Yang, Yuan Tian. The F-box gene Ppa promotes lipid storage in Drosophila [J]. Hereditas(Beijing), 2021, 43(6): 615-622.
[12]	Dingwei Peng, Ruiqiang Li, Wu Zeng, Min Wang, Xuan Shi, Jianhua Zeng, Xiaohong Liu, Yaoshen Chen, Zuyong He. Editing the cystine knot motif of MSTN enhances muscle development of Liang Guang Small Spotted pigs [J]. Hereditas(Beijing), 2021, 43(3): 261-270.
[13]	Na Wang, Zhilian Jia, Qiang Wu. RFX5 regulates gene expression of the Pcdhα cluster [J]. Hereditas(Beijing), 2020, 42(8): 760-774.
[14]	Guoling Li, Shanxin Yang, Zhenfang Wu, Xianwei Zhang. Recent developments in enhancing the efficiency of CRISPR/Cas9- mediated knock-in in animals [J]. Hereditas(Beijing), 2020, 42(7): 641-656.
[15]	Yingnan Chen, Jing Lu. Application of CRISPR/Cas9 mediated gene editing in trees [J]. Hereditas(Beijing), 2020, 42(7): 657-668.

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Applications of the CRISPR/Cas9 system in insects

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Recommended Articles

Metrics

Comments