蓝莓花青素通过下调p53基因DNA甲基化抑制口腔癌KB细胞增殖及诱导细胞凋亡

doi:10.3724/SP.J.1005.2014.0566

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HEREDITAS(Beijing) ›› 2014, Vol. 36 ›› Issue (6): 566-573.doi: 10.3724/SP.J.1005.2014.0566

• Research Articles • Previous Articles Next Articles

Blueberry anthocyanins induce G₂/M cell cycle arrest and apoptosis of oral cancer KB cells through down-regulation methylation of p53

Qi Chen, Shaowei Li, Yuchen Jia, Li Wang

Research Center of Molecular Biology, Inner Mongolia Medical University, Hohhot 010059, China

Received:2013-09-26 Revised:2014-03-20 Online:2014-06-20 Published:2014-05-28

Abstract

Abstract:

Blueberries are an excellent source of dietary polyphenols such as anthocyanins and phenolic acids. In this study, we investigated the ability of anthocyanins from the wild blueberries of Inner Mongolia to suppress the growth of the oral cancer cell line KB. The blueberry anthocyanins were extracted with methanol-containing 0.1% (v/v) hydrochloric acid. Fourteen unique anthocyanins were identified using high-performance liquid chromatography-mass spectrometry (HPLC-MS). The anticancer bioactivity of the extracts on KB cells was analyzed using methylthiazolyl-tetrazolium (MTT), flow cytometry (FCM) and immunocytochemistry. It was shown that the blueberry anthocyanins suppressed the proliferation of KB cells in a dose-dependent manner, as well as induced G₂/M cell cycle arrest and apoptosis of oral cancer KB cells. Immunocytochemistry analysis showed that the expression of caspase-9 and cytochrome c were obviously increased after the anthocyanins treatment. Western blot analysis also indicated that the expression of p53 was increased. Methylation-specific PCR (MSP) showed that the amount of unmethylated p53 increased, indicating that the anthocyanins can down-regulate the methylation of p53.

Key words: anthocyanins, anticancer, blueberry, oral cancer, DNA methylation

Qi Chen, Shaowei Li, Yuchen Jia, Li Wang. Blueberry anthocyanins induce G₂/M cell cycle arrest and apoptosis of oral cancer KB cells through down-regulation methylation of p53[J]. HEREDITAS(Beijing), 2014, 36(6): 566-573.

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Blueberry anthocyanins induce G₂/M cell cycle arrest and apoptosis of oral cancer KB cells through down-regulation methylation of p53

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Blueberry anthocyanins induce G2/M cell cycle arrest and apoptosis of oral cancer KB cells through down-regulation methylation of p53

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Blueberry anthocyanins induce G₂/M cell cycle arrest and apoptosis of oral cancer KB cells through down-regulation methylation of p53