CRISPR-Cas基因编辑技术在羊生产应用中研究进展

doi:10.16288/j.yczz.24-155

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Hereditas(Beijing) ›› 2024, Vol. 46 ›› Issue (9): 690-700.doi: 10.16288/j.yczz.24-155

• Review • Previous Articles Next Articles

Progress on CRISPR-Cas gene editing technology in sheep production

Dongxia Pan(), Hui Wang, Benhai Xiong, Xiangfang Tang()

State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2024-05-31 Revised:2024-07-15 Online:2024-09-20 Published:2024-08-15
Contact: Xiangfang Tang E-mail:pandongxia1117@163.com;xiangfangtang@163.com
Supported by:
National Key Research and Development Program of China “Demonstration of Large-scale Efficient and Healthy Breeding of Cattle and Sheep”(2022YFD1301100)

Abstract

Abstract:

Gene editing is a kind of genetic engineering technology that can modify the genome. In recent years, with the rapid development of molecular biotechnology, the clustered regularly interspaced short palindromic repeats associated protein system has been widely used as a powerful gene editing tool due to its high efficiency, accuracy and flexibility. The CRISPR-Cas system makes a significant contribution to different aspects of livestock production by introducing site-specific modifications such as insertions, deletions or single base replacements at specific genomic sites. In terms of sheep production applications, by establishing animal models that improve production economic traits and disease resistance, the function of key genes can be studied to accelerate the improvement of traits, thereby accelerating the improvement of traits. In this review, we summarize the mechanism and function of CRISPR-Cas system and its application in the production of reproductive traits, meat use traits, wool production traits, lactation traits and disease resistance traits of sheep and the establishment of sheep animal models.

Key words: CRISPR-Cas system, gene editing technology, sheep, goats, production research

Dongxia Pan, Hui Wang, Benhai Xiong, Xiangfang Tang. Progress on CRISPR-Cas gene editing technology in sheep production[J]. Hereditas(Beijing), 2024, 46(9): 690-700.

Figures/Tables 1

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特性	Cas核酸酶
特性	Cas9	Cas9n	dCas9	Cas12a (Cpf1)	Cas12b (C2c1)
CRISPR-Cas系统分类	2类II-A型	2类II-A型	2类II-A型	2类V-A型	2类V-B型
核酸酶结构域	HNH和RuvC	HNH或RuvC	无	RuvC	RuvC
PAM	NGG/NAG/NGA	NGG	NGG	(T)TTN	TTN
引导RNA	tracrRNA+crRNA (~120 nt)	tracrRNA+crRNA (~120 nt)	tracrRNA+crRNA (~120 nt)	crRNA (40~44 nt)	tracrRNA+crRNA (~120 nt)
靶向目标	dsDNA	dsDNA	dsDNA	ds/ssDNA	ds/ssDNA
反式切割活性	ssDNA/ssRNA	ssDNA/ssRNA	无	ssDNA	ssDNA

Progress on CRISPR-Cas gene editing technology in sheep production

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