小麦tae-MIR156前体基因的克隆及其靶基因TaSPL17多态性分析

doi:10.3724/SP.J.1005.2014.0592

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HEREDITAS(Beijing) ›› 2014, Vol. 36 ›› Issue (6): 592-602.doi: 10.3724/SP.J.1005.2014.0592

• Research Articles • Previous Articles Next Articles

Cloning of tae-MIR156 precursor gene and sequence polymorphisms of tae-miR156 targeted TaSPL17

Xia Liu^{1, 2}, Bin Zhang², Xinguo Mao², Ang Li², Meirong Sun², Ruilian Jing²

1. College of Bioengineering, Shanxi University, Taiyuan 030006, China;
2. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2014-01-09 Revised:2014-02-11 Online:2014-06-20 Published:2014-05-28

Abstract

Abstract:

Squamosa-promoter binding protein (SBP)-box genes, encoding plant-specific transcription factors, play an important role in plant development. Some members of the SBP-box gene family are regulated by miR156. In this study, we cloned the tae-MIR156 precursor gene, which could form a stem loop after its transcription. Sequence analysis showed that TaSPL3 and TaSPL17 had putative targets of tae-miR156 among the ten wheat SBP-box genes. The diploid donor species of hexaploid common wheat (Triticum aestivum, genome AABBDD), i.e., Triticum urartu (AA) UR209 and Aegilops speltoides Y2001 (SS, closely related to BB) possessed more than one copy of SPL17 (SPL17-A1, SPL17-A2 and SPL17-A3 from Triticum urartu; SPL17-B1, SPL17-B2 and SPL17-B3 from Aegilops speltoides), while Aegilops tauschii (DD) Ae38 only possessed one (SPL17-D). The identities between nucleotide sequences of SPL17-A2 and SPL17-B2, SPL17-A3 and SPL17-B3 or SPL17-D were higher than 99%. They were highly similar with the sequence of TaSPL17 in common wheat cultivars Chinese Spring, Hengguan 35 and Shuangfengshou. These genes might originate from a common ancestor and were highly conserved in the process of evolution. The target site of tae-miR156 in TaSPL17 was also highly conserved in two subgroups consisted of accessions with diverse spike number per plant and genetic background.

Key words: miR156, gene, polymorphism analysis, common wheat

Xia Liu, Bin Zhang, Xinguo Mao, Ang Li, Meirong Sun, Ruilian Jing. Cloning of tae-MIR156 precursor gene and sequence polymorphisms of tae-miR156 targeted TaSPL17[J]. HEREDITAS(Beijing), 2014, 36(6): 592-602.

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Cloning of tae-MIR156 precursor gene and sequence polymorphisms of tae-miR156 targeted TaSPL17

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