乳铁蛋白的理化性质及其重组表达系统的研究进展

doi:10.16288/j.yczz.15-146

遗传 ›› 2015, Vol. 37 ›› Issue (9): 873-884.doi: 10.16288/j.yczz.15-146

乳铁蛋白的理化性质及其重组表达系统的研究进展

庞晓楠^{1, 3}, 弘笑³, 魏璇², 陈喜文⁴, 刘佳², 陈德富^{1, 3}

1. 药物化学生物学国家重点实验室(南开大学),天津300071;
2. 上海科技大学免疫化学研究所,上海201210;
3. 南开大学生命科学学院遗传学和细胞生物学系,天津300071;
4. 南开大学生命科学学院生物化学与分子生物学系,天津300071;

收稿日期:2015-04-09 修回日期:2015-07-29 出版日期:2015-09-20 发布日期:2015-09-20
通讯作者: 刘佳,副研究员,研究方向：生物化学。E-mail: liujia@shanghaitech.edu.cn 陈德富,教授,博士生导师,研究方向：分子遗传学。E-mail: chendefu@nankai.edu.cn
作者简介:庞晓楠,博士研究生,专业方向：分子遗传学。Tel: 022-23508270;E-mail: fightingnk@163.com
基金资助:
药物化学生物学国家重点实验室开放基金(编号：201503017)、天津市应用基础与前沿技术研究计划重点项目(编号：112JCZDJC22900)和创新人才培养基金(编号：J1103503)资助

Research progress in physicochemical characteristics of lactoferrin and its recombinant expression systems

Xiaonan Pang^{1, 3}, Xiao Hong³, Xuan Wei², Xiwen Chen⁴, Jia Liu², Defu Chen^{1, 3}

1. State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin 300071, China;
2. Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China;
3. Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China;
4. Department of Biochemistry and Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071, China

Received:2015-04-09 Revised:2015-07-29 Online:2015-09-20 Published:2015-09-20

摘要/Abstract

摘要： 乳铁蛋白(Lactoferrin, Lf)是分子量大小约为80 kDa的铁离子结合糖蛋白,是转铁蛋白(Transferrin, Tf)家族的成员之一。其理化性质独特,具有抑菌、抗病毒、抗癌、免疫调节、调节铁离子的吸收等诸多生物学功能。获得高产且有生物活性的重组乳铁蛋白,并用于临床治疗,一直是研究热点。随着基因工程技术的发展,已获得多个可表达重组乳铁蛋白的表达系统。本文对乳铁蛋白的理化性质、生物学活性、临床研究以及目前的重组表达系统进行综述,以期为乳铁蛋白的临床应用提供参考。

关键词: 乳铁蛋白, 抑菌, 抗病毒, 免疫调节, 重组表达系统

Abstract: Lactoferrin is an iron-binding glycoprotein with a molecular weight of about 80 kDa that belongs to the transferrin family. Due to its unique physical and chemical properties, lactoferrin has a variety of biological functions including antibacterial, antiviral, anticancer, immunomodulatory activities and regulation of iron absorption. High-yield production of recombinant lactoferrin with biological activity and its application in clinical treatment have been a hot topic for long time. With the development of genetic engineering techniques, various expression systems have been developed to produce recombinant lactoferrin. In this review, we summarize physicochemical characteristics, biological activities, clinical studies and current recombinant expression systems of lactoferrin, in order to provide references for its clinical application.

Key words: lactoferrin, antibacterial, antiviral, immunomodulatory, recombinant expression system

庞晓楠, 弘笑, 魏璇, 陈喜文, 刘佳, 陈德富. 乳铁蛋白的理化性质及其重组表达系统的研究进展[J]. 遗传, 2015, 37(9): 873-884.

Xiaonan Pang, Xiao Hong, Xuan Wei, Xiwen Chen, Jia Liu, Defu Chen. Research progress in physicochemical characteristics of lactoferrin and its recombinant expression systems[J]. HEREDITAS(Beijing), 2015, 37(9): 873-884.

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乳铁蛋白的理化性质及其重组表达系统的研究进展

Research progress in physicochemical characteristics of lactoferrin and its recombinant expression systems

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