临床肺炎链球菌常见序列型青霉素耐药性的流行病学研究

doi:10.16288/j.yczz.16-142

遗传 ›› 2016, Vol. 38 ›› Issue (10): 940-947.doi: 10.16288/j.yczz.16-142

临床肺炎链球菌常见序列型青霉素耐药性的流行病学研究

高薇¹, 史伟¹, 陈长会², 文德年³, 田进², 姚开虎¹

1. 首都医科大学附属北京儿童医院、北京市儿科研究所,儿科学国家重点学科,教育部儿科重大疾病研究重点实验室,国家呼吸系统疾病临床医学研究中心,儿童呼吸道感染性疾病研究北京市重点实验室,北京 100045;
2. 酉阳土家族苗族自治县人民医院,重庆医科大学附属第一医院酉阳医院,重庆 409899;
3. 中江县人民医院,四川 618100

收稿日期:2016-04-21 修回日期:2016-07-11 出版日期:2016-10-20 发布日期:2016-10-20
作者简介:姚开虎,博士研究生,研究员,研究方向：儿童呼吸道感染常见病原菌的流行病学特征。田进,本科,副主任技师,研究方向：细菌耐药和个体化治疗。
基金资助:
国家自然课科学基金项目(编号：81371853)和重庆市医学科研计划项目(编号：20142221)资助

Epidemiological study on the penicillin resistance of clinical Streptococcus pneumoniae isolates identified as the common sequence types

Wei Gao¹, Wei Shi¹, Changhui Chen², Denian Wen³, Jin Tian², Kaihu Yao¹

1. Key Laboratory of Major Diseases in Children, Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Beijing 100045, China;
2. Youyang Hospital, The First Affiliated Hospital of Chongqing Medical University;
People's Hospital of Chongqing Youyang County, Chongqing 409899, China;
3. People's Hospital of Zhongjiang County, Sichuan 618100, China

Received:2016-04-21 Revised:2016-07-11 Online:2016-10-20 Published:2016-10-20
Supported by:
[Supported by the National Natural Science Foundation of China (No; 81371853) and Chongqing Medical Scientific Project (No.20142221)]

摘要/Abstract

摘要： 从菌种的水平研究和阐述临床分离的肺炎链球菌青霉素耐药机制存在一定的局限。为探讨以序列型(Sequence type,ST)为基础研究肺炎链球菌青霉素耐药机制的可行性,本研究分析了1997~2014年间北京常见STs肺炎链球菌488株和2015年重庆酉阳县、四川中江县常见STs菌株88株的青霉素最低抑菌浓度(Minimun inhibitory concentration,MIC)的分布及年份分布。结果显示北京分离株中除了ST342外,属于某一种ST的所有分离株的青霉素MIC值具有一定分布范围,或者<0.25 mg/L,或者≥0.25 mg/L。青霉素MIC <0.25 mg/L的分离株多分布于2001年以前,此年份后≥0.25 mg/L的分离株出现,并逐渐成为主要种群。但这个年份分布规律对于某一种ST并不明显,某一种ST在最初发现的几个年份中就具有不同青霉素MIC水平的分离株。重庆酉阳县和四川中江县常见STs型青霉素MIC分布于0.25~2.0 mg/L(≥0.25 mg/L),包括ST271、ST320和ST81。本研究从流行病学角度揭示了肺炎链球菌临床分离株常见STs的青霉素MIC值分布规律,支持以STs为基础研究其青霉素耐药机制。

关键词: 肺炎链球菌, 序列型, 耐药性, 青霉素

Abstract: There were some limitation in the current interpretation about the penicillin resistance mechanism of clinical Streptococcus pneumoniae isolates at the strain level. To explore the possibilities of studying the mechanism based on the sequence types (ST) of this bacteria, 488 isolates collected in Beijing from 1997-2014 and 88 isolates collected in Youyang County, Chongqing and Zhongjiang County, Sichuan in 2015 were analyzed by penicillin minimum inhibitory concentration (MIC) distribution and annual distribution. The results showed that the penicillin MICs of the all isolates covering by the given ST in Beijing have a defined range, either <0.25 mg/L or≥0.25 mg/L, except for the ST342. The isolates with penicillin MIC <0.25 mg/L were mainly collected before 2001, after which the isolates with MIC≥0.25 mg/L occurred and became the major population gradually. This law of year distribution, however, was not obvious for any specific ST. The isolates covering by any given ST could be determined with different penicillin MICs in the first few years after it was identified. The penicillin MIC of isolates identified as common STs and collected in Youyang County, Chongqing and Sichuan Zhongjiang County, including the ST271, ST320 and ST81, was around 0.25~2 mg/L (≥0.25 mg/L). Our study revealed the epidemiological distribution of penicillin MICs of the given STs determined in clinical S. pneumoniae isolates, suggesting that it is reasonable to research the penicillin resistance mechanism based on the STs of this bacteria.

Key words: Streptococcus pneumonia, sequence type, drug resistance, penicillin

高薇1, 史伟1, 陈长会2, 文德年3, 田进2, 姚开虎1. 临床肺炎链球菌常见序列型青霉素耐药性的流行病学研究[J]. 遗传, 2016, 38(10): 940-947.

Wei Gao, Wei Shi, Changhui Chen, Denian Wen, Jin Tian, Kaihu Yao. Epidemiological study on the penicillin resistance of clinical Streptococcus pneumoniae isolates identified as the common sequence types[J]. Hereditas(Beijing), 2016, 38(10): 940-947.

参考文献

[1] Hakenbeck R, Brückner R, Denapaite D, Maurer P. Molecular mechanisms of β-lactam resistance in Streptococcus pneumoniae . Future Microbiol , 2012, 7(3): 395-410.
[2] Zhou WQ, Yao KH, Zhang G, Yang YH, Li Y, Lv Y, Feng J. Mechanism for transfer of transposon Tn 2010 carrying macrolide resistance genes in Streptococcus pneumoniae and its effects on genome evolution. J Antimicrob Chemother , 2014, 69(6): 1470-1473.
[3] Zhang G, Wang C, Sui ZH, Feng J. Insights into the evolutionary trajectories of fluoroquinolone resistance in Streptococcus pneumoniae . J Antimicrob Chemother , 2015, 70(9): 2499-2506.
[4] Yao KH, Tong YJ, Yu SJ, Shen XZ, Yang YH. The relationship between penicillin-binding protein genes restriction polymorphism and penicillin susceptibility in Streptococcus pneumoniae . Chin J Lab Med , 2006, 29(12): 1107-1110. 姚开虎, 佟月娟, 俞桑洁, 沈叙庄, 杨永弘. 肺炎链球菌青霉素结合蛋白基因多态性与其青霉素敏感性的关系. 中华检验医学杂志, 2006, 29(12): 1107-1110.
[5] Li SY, Shen XZ, Wang YH, Yang YH. Alterations of murM gene and high-level penicillin-resistant as well as ceftriaxone resistance of Streptococcus pneumoniae . Chin J Microbiol Immunol , 2004, 24(10): 823-827. 李绍英, 沈叙庄, 王咏红, 杨永弘. murM基因变异与肺炎链球菌青霉素高度耐药及头孢曲松耐药的相关性. 中华微生物学和免疫学杂志, 2004, 24(10): 823-827.
[6] Li QH, Yao KH, Yu SJ, Ma X, He MM, Shi W, Yang YH. Spread of multidrug-resistant clonal complex 271 of serotype 19F Streptococcus pneumoniae in Beijing, China: characterization of serotype 19F. Epidemiol Infect , 2013, 141(12): 2492-2496.
[7] Xue L, Yao KH, Yu SJ, Liu ZJ, Qian J, Shen XZ, Yang YH. Molecular epidemiology of serotype 19A Streptococcus pneumoniae isolated from children in Beijing, 1997-2006. Chin Med J , 2011, 124(12): 1769-1774.
[8] Ma X, Yao KH, Yu SJ, Zhou L, Li QH, Shi W, He MM, Yang YH. Genotype replacement within serotype 23F Streptococcus pneumoniae in Beijing, China: characterization of serotype 23F. Epidemiol Infect , 2013, 141(8): 1690-1696.
[9] Shi W, Yao KH, He MM, Yu SJ, Yang YH. Population biology of 225 serogroup 6 Streptococcus pneumoniae isolates collected in China. BMC Infect Dis , 2014, 14(1): 467.
[10] He MM, Yao KH, Shi W, Gao W, Yuan L, Yu SJ, Yang YH. Dynamics of serotype 14 Streptococcus pneumoniae population causing acute respiratory infections among children in China (1997-2012). BMC Infect Dis , 2015, 15(1): 266.
[11] Sørensen UB. Typing of pneumococci by using 12 pooled antisera. J Clin Microbiol , 1993, 31(8): 2097-2100.
[12] Kelly LM, Jacobs MR, Appelbaum PC. Comparison of agar dilution, microdilution, E-test, and disk diffusion methods for testing activity of cefditoren against Streptococcus pneumoniae . J Clin Microbiol , 1999, 37(10): 3296-3299.
[13] Enright MC, Spratt BG. A multilocus sequence typing scheme for Streptococcus pneumoniae : identification of clones associated with serious invasive disease. Microbiology , 1998, 144(11): 3049-3060.
[14] Sanbongi Y, Ida T, Ishikawa M, Osaki Y, Kataoka H, Suzuki T, Kondo K, Ohsawa F, Yonezawa M. Complete sequences of six penicillin-binding protein genes from 40 Streptococcus pneumoniae clinical isolates collected in Japan. Antimicrob Agents Chemother , 2004, 48(6): 2244-2250.
[15] Sauerbier J, Maurer P, Rieger M, Hakenbeck R. Streptococcus pneumoniae R6 interspecies transformation: genetic analysis of penicillin resistance determinants and genome-wide recombination events. Mol Microbiol , 2012, 86(3): 692-706.
[16] Lin F, Zheng MQ, Zeng AP, Ding D, Wen SY, Wang SQ. Study on PBPs genes of penicillin-resistant Streptococcus pneumonia in Zhejiang province. Hereditas ( Beijing ), 2005, 27(6): 965-971. 林峰, 郑敏巧, 曾爱平, 丁玎, 文思远, 王升启. 浙江地区青霉素耐药肺炎链球菌PBPs基因及氨基酸序列研究. 遗传, 2005, 27(6): 965-971.
[17] Calix JJ, Porambo RJ, Brady AM, Larson TR, Yother J, Abeygunwardana C, Nahm MH. Biochemical, genetic, and serological characterization of two capsule subtypes among Streptococcus pneumoniae Serotype 20 strains: discovery of a new pneumococcal serotype. J

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

临床肺炎链球菌常见序列型青霉素耐药性的流行病学研究

Epidemiological study on the penicillin resistance of clinical Streptococcus pneumoniae isolates identified as the common sequence types

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

[1]	陈湖星, 徐蕾, 李静, 郭政, 敖露. 基于结直肠癌细胞系MIC₅₀相关基因对的基础耐药评分模型构建[J]. 遗传, 2020, 42(6): 577-585.
[2]	祝力骋,卢俊婉,王建,许腾,徐娟华. 肺炎克雷伯菌bla_CARB-2基因的分布及结构分析[J]. 遗传, 2018, 40(7): 593-600.
[3]	杨盛智, 吴国艳, 龙梅, 邓雯文, 王红宁, 邹立扣. 鸡蛋生产链中沙门氏菌对抗生素及消毒剂的耐药性研究[J]. 遗传, 2016, 38(10): 948-956.
[4]	陈昱帆, 刘诗胤, 梁志彬, 吕明发, 周佳暖, 张炼辉. 群体感应与微生物耐药性[J]. 遗传, 2016, 38(10): 881-893.
[5]	高琼,黄海辉. 艰难梭菌耐药性及耐药机制研究进展[J]. 遗传, 2015, 37(5): 458-464.
[6]	杨延成,程航,周人杰,饶贤才. SCCmec遗传元件及其在耐甲氧西林金黄色葡萄球菌分子分型中的应用[J]. 遗传, 2015, 37(5): 442-451.
[7]	张跃进, 常清利, 汪倩, 卢俊婉, 王欢, 李佩珍, 应俊, 包其郁, 胡云良. 临床分离肺炎克雷伯菌Ⅰ类整合子的结构与功能[J]. 遗传, 2014, 36(6): 603-610.
[8]	郭云学，莫德林，陈瑶生，张悦，张云，肖书奇，刘小红. PI3K/AKT抑制剂渥曼青霉素对猪前体脂肪细胞增殖和凋亡的影响[J]. 遗传, 2012, 34(10): 1282-1290.
[9]	王倩，崔志峰. 真菌的多向耐药性ABC转运蛋白[J]. 遗传, 2011, 33(10): 1048-1056.
[10]	张春玉，冯源熙，李，璞，傅松滨. 介导多药耐药的ABC转运蛋白超家族与MTX耐药性的关系研究[J]. 遗传, 2006, 28(10): 1201-1205.
[11]	林峰，郑敏巧，曾爱平，丁玎，文思远，王升启. 浙江地区青霉素耐药肺炎链球菌PBPs基因及氨基酸序列研究[J]. 遗传, 2005, 27(6): 965-971.