鸡蛋生产链中沙门氏菌对抗生素及消毒剂的耐药性研究

doi:10.16288/j.yczz.16-185

遗传 ›› 2016, Vol. 38 ›› Issue (10): 948-956.doi: 10.16288/j.yczz.16-185

• 研究报告 • 上一篇

鸡蛋生产链中沙门氏菌对抗生素及消毒剂的耐药性研究

杨盛智¹, 吴国艳¹, 龙梅¹, 邓雯文¹, 王红宁², 邹立扣¹

1. 四川农业大学资源学院,成都 611130;
2. 四川大学动物疫病防控与食品安全四川省重点实验室,成都 610064

收稿日期:2016-05-21 修回日期:2016-07-21 出版日期:2016-10-20 发布日期:2016-10-20
作者简介:邹立扣,博士,教授,博士生导师,研究方向：微生物与食品安全。
基金资助:
国家自然科学基金项目(编号：31671954)资助

Antibiotic and disinfectant resistance of Salmonella isolated from egg production chains

Shengzhi Yang¹, Guoyan Wu¹, Mei Long¹, Wenwen Deng¹, Hongning Wang², Likou Zou¹

1. College of Resources, Sichuan Agricultural University, Chengdu 611130, China;
2. Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, School of Life Science, Sichuan University, Chengdu 610064, China

Received:2016-05-21 Revised:2016-07-21 Online:2016-10-20 Published:2016-10-20
Supported by:
[Supported by the National Natural Science Foundation of China (No; 31671954)]

摘要/Abstract

摘要： 为研究鸡蛋生产链中沙门氏菌的污染情况及抗生素、消毒剂耐药情况,本文鉴定了鸡蛋生产链中分离得到的111株沙门氏菌(Salmonella)血清型,并测定了抗生素和消毒剂对沙门氏菌的最小抑菌浓度(Minimum inhibitory concentrations, MICs),检测了其对抗生素和消毒剂的耐药基因的表达情况。研究结果表明,沙门氏菌对甲氧苄啶(Trimethoprim, TMP)耐药率最高(N=100,P=90.09%),对阿莫西林/克拉维酸(Amoxicillin and clavulanate, AMC)、头孢噻呋钠(Sodium ceftiofur, CFS)、庆大霉素(Gentamicin, CN)敏感。沙门氏菌共产生6种不同的耐药谱型,TMP是最主要的耐药谱型(N=36,P=32.43%),52.25%的菌株(N=58)具有多重耐药性。苯扎氯铵(Benzalkonium chloride, BC)与氯化十六烷基吡啶(Cetylpyridinium chloride, CPC)对沙门氏菌的MIC的范围分别为：8~128 μg/mL、8~256 μg/mL。相对于质控菌株Escherichia coli ATCC10536,101株沙门氏菌对BC和CPC同时具有较高的耐药性(P=90.99%),109株沙门氏菌对抗生素和消毒剂具有共同耐药性(P=98.20%)。抗生素耐药基因检出率最高为blaTEM(N=49, P=44.14%),未检测出qnrA、qnrB、qepA基因,仅检测出qacEΔ1消毒剂耐药基因(N=63, P=56.76%)。抗生素耐药基因sul1和消毒剂耐药基因qacEΔ1具有显著相关性(P<0.01)。S. Derby对TMP、土霉素(Oxytetracycline, OTC)、阿莫西林(Amoxicillin, AML)、环丙沙星(Ciprofloxacin, CIP)同时表现较高的耐药性,S. Derby检出了11种抗生素耐药基因,消毒剂耐药基因qacEΔ1的检出率为81.25%(N=52)。鸡场中养殖内环境沙门氏菌对抗生素和消毒剂的耐药率以及耐药基因检出率均高于养殖外环境,鸡蛋包装、储存及销售等环节中沙门氏菌耐药率及耐药基因检出率均较高。由此可见,鸡蛋生产链中沙门氏菌对抗生素、消毒剂耐药性较严重,且存在共同耐药的现象。因此,需要进一步规范防控鸡场中沙门氏菌,规范抗生素和消毒剂的使用以及加强鸡蛋生产链条中卫生安全的监管。

关键词: 沙门氏菌, 抗生素, 消毒剂, 耐药性, 鸡蛋生产链

Abstract: To investigate the contamination of Salmonella and its drug resistance in egg production chains, 111 Salmonella strains of different serotypes isolated from egg production chains were used in the study. The minimum inhibitory concentrations (MICs) of antibiotics and disinfectants against Salmonella isolates were determined, meanwhile, antibiotic and disinfectant resistance genes were amplified. The results showed that the resistance frequency of trimethoprim (TMP, N=100, P=90.09%) was highest among Salmonella isolates and all isolates were sensitive to amoxicillin and clavulanate (AMC), ceftiofur sodium (CFS) and gentamicin (CN), respectively. There were six different antibiotic resistance profiles, and TMP profile was the most prevalent type (N=36, P=32.43%). 52.25% of Salmonella isolates appeared multi-drug resistance. The MICs of benzalkonium chloride (BC) and cetylpyridinium chloride (CPC) against Salmonella strains ranged from 8 to 128 μg/mL and 8 to 256 μg/mL, respectively. Compared to quality control strain Escherichia coli ATCC10536, 101 Salmonella isolates (P=90.99%) had dual resistances to BC and CPC. 109 Salmonella (P=98.20%) were co-resistant to antibiotic and disinfectant. Detection of drug resistance genes showed that blaTEM gene was dominant (N=49, P=44.14%). The qnrA, qnrB and qepA genes were not detected. Only qacEΔ1 gene (N=63, P=56.76%) was detected among the disinfectant resistance genes. There was a significant correlation between sul1 gene and qacEΔ1 gene (P < 0.01). S. Derby showed multi-resistances to TMP, oxytetracycline (OTC), amoxicillin (AML) and ciprofloxacin (CIP). Eleven antibiotic resistance genes were found in S. Derby, in which the prevalence of qacEΔ1 gene was 81.25% (N=52). Besides, the drug resistance frequency and the prevalence of drug resistance genes in internal farm environment were higher than those in external environment. High frequency of drug resistances and high prevalence of drug resistance genes were detected in all links of the egg production chains, including package, storage and sale. Our results showed that severe antibiotic and disinfectant resistances existed in egg production chains. Therefore, further hygiene supervision should be implemented to prevent and control Salmonella, and standardize the use of antibiotics and disinfectants.

Key words: Salmonella, antibiotic, disinfectant, resistance, egg production chains

杨盛智, 吴国艳, 龙梅, 邓雯文, 王红宁, 邹立扣. 鸡蛋生产链中沙门氏菌对抗生素及消毒剂的耐药性研究[J]. 遗传, 2016, 38(10): 948-956.

Shengzhi Yang, Guoyan Wu, Mei Long, Wenwen Deng, Hongning Wang, Likou Zou. Antibiotic and disinfectant resistance of Salmonella isolated from egg production chains[J]. Hereditas(Beijing), 2016, 38(10): 948-956.

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鸡蛋生产链中沙门氏菌对抗生素及消毒剂的耐药性研究

Antibiotic and disinfectant resistance of Salmonella isolated from egg production chains

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