利用全基因组重测序分析鹿茸重量相关基因

doi:10.16288/j.yczz.17-116

遗传 ›› 2017, Vol. 39 ›› Issue (11): 1090-1101.doi: 10.16288/j.yczz.17-116

利用全基因组重测序分析鹿茸重量相关基因

胡鹏飞¹(),徐佳萍¹,艾成²,邵秀娟²(),王洪亮¹,董依萌³,崔学哲¹,杨福合¹,邢秀梅¹

1. 中国农业科学院特产研究所,特种经济动物分子生物学国家重点实验室,农业部特种经济动物遗传育种殖重点实验室,长春 130112
2. 中国农业科学院深圳农业基因组研究所,深圳 518120
3. 吉林农业大学,长春 130118

收稿日期:2017-03-30 修回日期:2017-06-11 出版日期:2017-11-20 发布日期:2017-12-20
作者简介:胡鹏飞,博士,副研究员,研究方向：梅花鹿产茸性能评价及分子调控机制。E-mail: pfhoo@hotmail.com|邢秀梅，博士，研究员，研究方向：特种经济动物种质资源保护与遗传育种。E-mail: xingxiumei2004@126.com
基金资助:
吉林省科技发展计划项目(20150309006YY)

Screening weight related genes of velvet antlers by whole genome re-sequencing

Pengfei Hu¹(),Jiaping Xu¹,Cheng Ai²,Xiujuan Shao²(),Hongliang Wang¹,Yimeng Dong³,Xuezhe Cui¹,Fuhe Yang¹,Xiumei Xing¹

1. State Key Laboratory for Molecular Biology of Special Economic Animals, Key Laboratory of Special Economic Animal Genetics and Breeding, Ministry of Agriculture, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China
2. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
3. Jilin Agricultural University, Changchun 130118, China

Received:2017-03-30 Revised:2017-06-11 Online:2017-11-20 Published:2017-12-20
Supported by:
the Science and Technology Development Project of Jilin Province(20150309006YY)

摘要/Abstract

摘要：

茸角是鹿科动物特有的器官,具有重要的生物学意义。鹿茸生长是一个复杂的生物代谢过程,其重量与遗传因素有一定关联。本研究对饲养条件基本一致的5个梅花鹿(Cervus nippon)群体进行调查,获得高产和低产梅花鹿个体共100只,利用全基因组重测序分析这些个体与鹿茸重量相关的遗传变异。结果表明,共得到94个与鹿茸重量可能相关的遗传变异,其中有2个变异位点分别定位于OAS2和ALYREF/THOC4基因的外显子区,且ALYREF/THOC4基因在鹿茸中表达量很高。功能富集分析发现,这些遗传变异与鹿茸生长发育密切相关,可作为潜在的鹿茸重量相关遗传变异。本研究首次通过全基因组重测序直接筛选与鹿茸重量相关的遗传变异,并分析关联基因的生物学功能,对揭示鹿茸生长发育和鹿茸重量差异形成的遗传机制具有重要意义。

关键词: 全基因组重测序, 梅花鹿, 鹿茸重量, 全基因组关联分析

Abstract:

The velvet antler is a special organ that has important biological significance for deer, and its growth is a complicated biological metabolism process. Growing evidence suggests that genetics factors play essential roles in the weight of velvet antlers. In this study, we investigated five sika deer (Cervus nippon) populations under the same feeding condition, and screened genetic variations in the 100 samples (including 50 heavy and 50 light velvet antler weight samples) by whole genome re-sequencing. The results showed that 94 genetic variations were related to the velvet antler weight, among which two single nucleotide polymorphism (SNP) sites were located on the exon regions of OAS2 and ALYREF/THOC4, respectively. Furthermore, ALYREF/THOC4 is highly expressed in the velvet antler. The biological functions of these genetic variations were highly related to the growth and development of deer velvet antlers. Collectively, we screened genes related to the velvet antler weight in sika deer populations by whole genome re-sequencing and identified 94 sites as candidate genetic variations related to the velvet antler weight. We hope that it will contribute to further mechanistic studies of velvet antler development and weight variations.

Key words: whole genome re-sequencing, Cervus nippon, velvet antler weight, genome wide association study

胡鹏飞, 徐佳萍, 艾成, 邵秀娟, 王洪亮, 董依萌, 崔学哲, 杨福合, 邢秀梅. 利用全基因组重测序分析鹿茸重量相关基因[J]. 遗传, 2017, 39(11): 1090-1101.

Pengfei Hu, Jiaping Xu, Cheng Ai, Xiujuan Shao, Hongliang Wang, Yimeng Dong, Xuezhe Cui, Fuhe Yang, Xiumei Xing. Screening weight related genes of velvet antlers by whole genome re-sequencing[J]. Hereditas(Beijing), 2017, 39(11): 1090-1101.

参考文献

[1]	Lincoln GA. Appearance of antler pedicles in early foetal life in red deer. J Embryol Exp Morphol, 1973, 29(2): 431-437.
[2]	Hartwig H, Schrudde J. Experimentelle untersuchungen zur bildung der prim?ren stirnauswüchse beim reh (Capreolus capreolus L.). Z Jagdwiss, 1974, 20(1): 1-13.
[3]	Yue ZP, Deng XM, Feng HH. The mechanisms of deer antlers development and regeneration. J Econ Anim, 2005, 9(1): 46-49
[3]	岳占碰, 邓旭明, 冯海华. 鹿茸角发育与再生机理. 经济动物学报, 2005, 9(1): 46-49.
[4]	赵肯堂. 角的形态、性别、生长和意义. 生物学通报, 1958, (12): 8-11.
[5]	蒋志刚. 生物进化的原动力——性选择. 科学中国人, 2002, (6): 54-56.
[6]	Williams JD, Krueger WF, Harmel DH. Heritabilities for antler characteristics and body weight in yearling white- tailed deer. Heredity, 1994, 73(1): 78-83.
[7]	Pérez-González J, Carranza J, Torres-Porras J, Fernández- García JL. Low heterozygosity at microsatellite markers in Iberian Red Deer with small antlers. J Hered, 2010, 101(5): 553-561.
[8]	Price JS, Allen S, Faucheux C, Althnaian T, Mount JG. Deer antlers: a zoological curiosity or the key to understanding organ regeneration in mammals?. J Anat, 2005, 207(5): 603-618.
[9]	Li CY, Zhao HP, Liu Z, McMahon C. Deer antler-a novel model for studying organ regeneration in mammals. Int J Biochem Cell Biol, 2014, 56: 111-122.
[10]	Sui ZG, Zhang LH, Huo YS, Zhang YK. Bioactive components of velvet antlers and their pharmacological properties. J Pharm Biomed Anal, 2014, 87: 229-240.
[11]	Tseng SH, Sung CH, Chen LG, Lai YJ, Chang WS, Sung HC, Wang CC. Comparison of chemical compositions and osteoprotective effects of different sections of velvet antler. J Ethnopharmacol, 2014, 151(1): 352-360.
[12]	林仲凡. 有关鹿茸( 角)的生物学研究进展. 国外畜牧学(草食家畜), 1985, (4): 7-10.
[13]	Kruuk LEB, Slate J, Pemberton JM, Brotherstone S, Guinness F, Clutton-Brock T. Antler size in red deer: heritability and selection but no evolution. Evolution, 2002, 56(8): 1683-1695.
[14]	李和平. 梅花鹿优良品种(品系)产茸性能研究. 中国畜牧杂志, 2003, 39(2): 31-32.
[15]	Li HP, Zheng XT, Bing GL, Li S, Zhao M, Chen XJ. Specific character analysis on artificial breed and strain of deer in China,. Hereditas(Beijing) 1997, 19(S1): 76-78.
[15]	李和平, 郑兴涛, 邴国良, 李生, 赵蒙, 陈学进. 中国茸鹿人工培育品种(品系)种质特性分析. 遗传, 1997, 19(S1): 76-78.
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利用全基因组重测序分析鹿茸重量相关基因

Screening weight related genes of velvet antlers by whole genome re-sequencing

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