遗传 ›› 2021, Vol. 43 ›› Issue (12): 1159-1169.doi: 10.16288/j.yczz.21-298

• 研究报告 • 上一篇    下一篇

玉米盐胁迫相关性状全基因组关联分析及候选基因预测

单婷玉(), 施雯, 王翌婷, 曹孜怡, 汪保华, 方辉()   

  1. 南通大学生命科学学院,农业农村部南方平原玉米科学观测实验站,南通 226019
  • 收稿日期:2021-08-13 修回日期:2021-10-02 出版日期:2021-12-20 发布日期:2021-12-07
  • 通讯作者: 方辉 E-mail:shan_0822@126.com;fanghui8912@126.com
  • 作者简介:单婷玉,在读硕士研究生,专业方向:玉米盐胁迫响应研究。E-mail: shan_0822@126.com
  • 基金资助:
    南通市科技项目和南通大学人才引进项目资助(MS22020033);南通大学人才引进项目资助编号(135420609055)

Genome-wide association study and candidate gene prediction of salt tolerance related traits in maize

Shan Tingyu(), Shi Wen, Wang Yiting, Cao Ziyi, Wang Baohua, Fang Hui()   

  1. Ministry of Agricultural Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region, School of Life Sciences, Nantong University, Nantong 226019, China
  • Received:2021-08-13 Revised:2021-10-02 Online:2021-12-20 Published:2021-12-07
  • Contact: Fang Hui E-mail:shan_0822@126.com;fanghui8912@126.com
  • Supported by:
    Supported by the Science and Technology Project of Nantong City, China(MS22020033);Talent Introduction Project of Nantong University No(135420609055)

摘要:

盐胁迫是影响玉米产量的重要因素,为探究玉米盐胁迫响应的遗传基础,本研究以150份遗传背景丰富的玉米自交系为材料,结合34,342个多态性SNP标记,利用混合线性模型对玉米两个盐胁迫相关性状进行全基因组关联分析。关联分析结果表明:共鉴定8个独立位点与盐胁迫相关性状显著关联,其中3个位点与枯萎度显著关联,分布在4号和9号染色体上,5个SNP位点与株高变化率显著关联,分布在1、2、3和6号染色体上。结合盐胁迫下基因的表达量数据和功能注释,筛选到11个候选基因,利用qRT-PCR验证其中7个基因在盐胁迫下表达量显著上调。本研究结果为玉米耐盐机理的解析奠定了基础,为玉米耐盐种质的培育提供新的靶基因。

关键词: 玉米, 盐胁迫, 混合线性模型, 全基因组关联分析

Abstract:

Salt stress is an important factor that affects maize yield. In order to explore the genetic basis of salt tolerance in maize, a genome-wide association study (GWAS) using the mixed liner model was conducted on 150 maize inbred lines with rich genetic background and 34,342 polymorphism SNP markers. A total of 8 independent loci were identified that significantly associated with salt-tolerance traits, among which 3 loci were significantly associated with withering degree on chromosomes 4 and 9; and the remaining 5 loci were significantly associated with plant height change rate on chromosomes 1, 2, 3 and 6. Eleven candidate genes were identified according to the gene expression data under salt stress; and functional annotations verified 7 of them to be significantly up-regulated under salt stress by qRT-PCR. These findings lay a foundation for understanding the mechanism(s) of maize salt tolerance and provide new target genes for the breeding of maize salt tolerance germplasm.

Key words: maize, salt tolerance, mixed liner model, genome-wide association study