遗传 ›› 2019, Vol. 41 ›› Issue (12): 1138-1147.doi: 10.16288/j.yczz.19-243

• 研究报告 • 上一篇    下一篇

大熊猫源致病大肠杆菌CCHTP全基因组测序及耐药和毒力基因分析

邓雯文1, 李才武1, 赵思越2, 李仁贵1, 何永果1, 吴代福1, 杨盛智2, 黄炎1, 张和民1, 邹立扣2()   

  1. 1. 中国大熊猫保护研究中心,大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室,都江堰 611830
    2. 四川农业大学资源学院,成都 611130
  • 收稿日期:2019-09-17 修回日期:2019-11-25 出版日期:2019-12-20 发布日期:2019-11-27
  • 通讯作者: 邹立扣 E-mail:zoulikou@sicau.edu.cn
  • 作者简介:邓雯文,硕士研究生,研究方向:大熊猫分子遗传学。E-mail: dwenwen130@163.com|李才武,硕士研究生,高级工程师,研究方向:大熊猫临床兽医。E-mail: 83330019@qq.com; 邓雯文和李才武并列第一作者。
  • 基金资助:
    中国大熊猫保护研究中心项目编号(CCRCGP181918);国家自然科学基金项目资助编号(31400066)

Whole genome sequencing reveals the distribution of resistance and virulence genes of pathogenic Escherichia coli CCHTP from giant panda

Deng Wenwen1, Li Caiwu1, Zhao Siyue2, Li Rengui1, He Yongguo1, Wu Daifu1, Yang Shengzhi2, Huang Yan1, Zhang Hemin1, Zou Likou2()   

  1. 1. China Conservation and Research Centre of the Giant Panda, Key Laboratory of SFGA on Conservation Biology of Rare Animals in the Giant Panda National Park, Dujiangyan 611830, China
    2. Faculty of Applied Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2019-09-17 Revised:2019-11-25 Online:2019-12-20 Published:2019-11-27
  • Contact: Zou Likou E-mail:zoulikou@sicau.edu.cn
  • Supported by:
    Supported by the Project of China Conservation and Research Center for the Giant Panda No(CCRCGP181918);the National Natural Science Foundation of China No(31400066)

摘要:

致病性大肠杆菌是引起动物泌尿系统感染的重要病原菌,本研究对泌尿生殖道感染出现潜血的大熊猫尿液中分离的一株致病性大肠杆菌(Escherichia coli CCHTP)进行全基因组测序,检测其中耐药基因和毒力因子的情况,同时对基因岛上耐药和毒力基因及其基因环境进行研究。研究发现,大肠杆菌CCHTP中存在多种类型的耐药基因,其中外排泵系统基因数量最多,包括mdfA、emrE和mdtN等介导多重耐药外排泵的基因。此外,该菌还携带166种毒力因子及563个相关毒力基因,其中属于黏附与侵袭类的毒力因子及相关基因数量最多。对19个基因岛分析发现,基因岛GIs011和GIs017中各有一段包含耐药和毒力基因的序列,两侧与可移动遗传元件(转座酶和插入序列)相连,这些结构可能介导耐药及毒力基因水平转移。本研究通过全基因组测序分析了大熊猫源致病性大肠杆菌中存在的耐药及毒力基因情况,对大熊猫相关疾病的科学治疗、合理用药有重要意义。

关键词: 大熊猫, 大肠杆菌, 全基因组测序, 耐药基因, 毒力因子

Abstract:

Pathogenic Escherichia coli (E. coli) is the most common pathogen causing urinary tract infection in animals. We investigated the antibiotic resistance and virulence genes of pathogenic E. coli CCHTP derived from urine with occult blood of the giant panda by whole genome sequencing. The flanking sequencing of resistance and virulence genes in genomic islands were also analyzed. Our results demonstrate that E. coli CCHTP contains different families of antibiotic resistance genes, most of which are efflux pump related genes, including multiple drug resistance efflux pump genes mdfA, emrE, and mdtN. A total of 166 virulence factors and 563 virulence genes were identified, and the most virulence factors and related genes are involved in host cell attachment and invasion processes. Furthermore, sequence analysis of 19 genomic islands revealed that antibiotic and virulence genes are associated with mobile genetic elements (transposon and insertion sequence) in GIs011 and GIs017. These structures can mediate horizontal transfer of antibiotic and virulence genes. Our work described the distribution of antibiotic resistance genes and virulence genes in E. coli CCHTP, which may provide an important guidance for treatment and rational drug use of E. coli CCHTP infection in the giant panda.

Key words: giant panda, Escherichia coli, whole genome sequencing, resistance gene, virulence gene