关键词: 趋化因子受体-2, 基因多态性, 人类免疫缺陷病毒1

Abstract:

The purpose of the work is to investigate the frequencies and polymorphisms of HIV-1 resistant CCR2-64Ⅰ alleles in 14 Chinese populations in South China. The blood samples include 791 unrelated healthy individuals. Whole blood samples were collected randomly and their genomic DNA were extracted with OMEGA Blood Kits. Allelic frequencies were identified by PCR-RFLP analysis. 636 individuals were wild-type homozygous, 104 individuals were CCR2-64Ⅰ heterozygote and 51 individuals were CCR2-64Ⅰ homozygous. Distributions of the CCR2-64Ⅰalleles among the popula-tions were in accordance with Hardy-Weinberg equilibrium. The average allele frequencies was 13.6%, and the frequency span of the alleles was 1.6%-30.3%. By statistical analysis, the frequency varied significantly in 14 populations (P<0.05). Among them, Zhuang ethnic population had the lowest CCR2-64Ⅰallele, whereas the Lisu ethnic population in Yunnan province had the highest CCR2-64Ⅰallele Conclusion: The frequency of CCR2-64Ⅰallele of 12 ethnic populations were lower than that of Han. The frequency of CCR2-64Ⅰallele between Southern and South-east group showed significant variations. The mutant alleles conferring genetic resistance to HIV-1 and AIDS progression needs to be further studied.