遗传 ›› 2009, Vol. 31 ›› Issue (8): 855-858.doi: 10.3724/SP.J.1005.2009.00855

• 研究报告 • 上一篇    下一篇

陆地棉亚红株突变性状的连锁遗传规律分析

陈旭升;狄佳春;马晓杰;刘新民   

  1. 江苏省农业科学院经济作物研究所, 南京210014
  • 收稿日期:2008-12-24 修回日期:2009-02-10 出版日期:2009-08-10 发布日期:2009-08-10
  • 通讯作者: 陈旭升

Analysis on linkage inheritance of sub-red plant mutant from upland cotton

CHEN Xu-Sheng, DI Jia-Chun, MA Xiao-Jie, LIU Xin-Min   

  1. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2008-12-24 Revised:2009-02-10 Online:2009-08-10 Published:2009-08-10
  • Contact: CHEN Xu-Sheng

摘要: 以具有绿叶、花瓣红心的陆地棉、海岛棉与具有亚红株、无红心的陆地棉种质为试验材料, 通过经典遗传学的二点测验分析了亚红株突变的连锁遗传规律; 结果显示, 测交群体(E083×B026)×苏9701、(B026×E083)×苏9701的平均交换值为1.35%; 测交群体(E083×海7124)×苏9701、(海7124×E083)×苏9701的平均交换值为2.94%。同时, 用亚红株、无红心、棕絮与绿叶、红心、白絮种质杂交, 进行三点测验, 结果表明: 基因Rs在Lc1、R2之间; 基因Rs和Lc1的遗传距离为42.21 cM, RsR2的遗传距离为 1.68 cM。当两对基因同时发生双交换时, 相互干扰小, 符合系数达0.79。根据已知的基因遗传距离, 整合后绘制了遗传连锁图: Rs 基因位于基因R2、Lc1以及分子标记NAU2863、NAU3735和NAU1048、BNL2634之间; 其中Rs基因两侧的分子标记NAU3735和NAU1048与它的遗传距离分别为0.1 cM和0.2 cM。

关键词: 陆地棉, 亚红株突变, 遗传距离, 连锁图

Abstract: Analysis of linkage inheritance of sub-red plant, a natural mutant from upland cotton, was conducted by two-point test using parents Gossypium barbadense Hai7124 and G. hirsutum B026 both with petal spots and green leaves, and G. hirsutum E083 with normal white flowers and, sub-red plants. The average percentage of recombination was 1.35% when calculating test-cross combining populations derived from E083/B026//Su9701 and B026/E083//Su9701. The average percentage of recombination was 2.94% when calculating test-cross combining populations derived from E083/Hai7124// Su9701 and Hai7124/E083//Su9701. Meanwhile, the genetic stocks having genotype Rs, Lc1, r2, and rs, lc1, and R2 were used in the three-point test, which demonstrated that the mutant gene Rs was located between genes Lc and R2. The genetic distance between Rs and Lc1 was 42.21 cM, and 1.68 cM between Rs and R2. Coefficient of interference among R2, Lc1, and Rs was 0.79, indicating that the single cross between Rs and R2 has less impact on the other single cross between Rs and Lc1. Based on known genetic distance around gene Rs, the genetic linkage map was drawn. Rs is located between genes Lc1 and R2, molecular marker NAU2863, NAU3735 and NAU3735, NAU1048. The genetic distances between Rs and two markers NAU3735, NAU1048 are 0.1 and 0.2 cM, respectively.