利用向日葵重组自交系构建遗传图谱

doi:10.3724/SP.J.1005.2014.1036

遗传 ›› 2014, Vol. 36 ›› Issue (10): 1036-1042.doi: 10.3724/SP.J.1005.2014.1036

利用向日葵重组自交系构建遗传图谱

张永虎¹,于海峰²,侯建华¹,李素萍²,吕品¹,于志贤¹

1. 内蒙古农业大学农学院,呼和浩特 010018;
2. 内蒙古自治区农牧业科学院,呼和浩特 010031

收稿日期:2014-05-23 出版日期:2014-10-20 发布日期:2014-10-20
通讯作者: 侯建华,博士,博士生导师,研究方向：作物抗逆性状的遗传改良。E-mail: Houjh68@163.com E-mail:zhangyonghu0815@126.com
作者简介:张永虎,博士研究生,研究方向：作物抗性遗传改良。E-mail: zhangyonghu0815@126.com
基金资助:
国家自然科学基金项目(编号：31160288)和内蒙古自然科学基金重点项目(编号：2010Zd06)资助

Construction of a genetic map of sunflower using a population of recombinant inbred lines (RILs)

Yonghu Zhang¹, Haifeng Yu², Jianhua Hou¹, Suping Li², Pin Lv¹, Zhixian Yu¹

1. Agricultural College, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Huhhot 010031, China

Received:2014-05-23 Online:2014-10-20 Published:2014-10-20

摘要/Abstract

摘要： 以向日葵自选系K55为母本、K58为父本杂交组合,通过单粒传得到的187个F_5：6代重组自交系群体为作图材料,联合应用SSR和AFLP标记构建遗传连锁图谱。经过78对SSR引物和48对AFLP引物组合选择性扩增,分别得到341和1119条带,共1460条,分别获得多态性条带184条和393条,共577条多态性条带,占所有条带的39.52%。SSR和AFLP标记各有84个和108个多态性标记偏离孟德尔分离比例(P=0.05),共192个偏分离标记。采用JoinMap4.0软件进行连锁分析,构建了1张总长度为2759.4 cM、包含17个连锁群、连锁495个多态性标记的遗传图谱,其中偏分离标记170个,标记间的平均图距为5.57 cM。每个连锁群上分布有5~72个标记,长68.88~250.17 cM。本图谱为向日葵永久性图谱,为向日葵重要性状QTL定位和基因克隆奠定基础。

关键词: 向日葵, 重组自交系, SSR, AFLP, 分子连锁图谱

Abstract: A genetic linkage map of sunflower was constructed by combined applying the SSR and AFLP markers using 187 F_5:6 individuals of recombinant inbred lines (RILs) which derived from the cross between Helianthus annuus K55 and Helianthus annuus K58 through single-seed descent (SSD). Using 78 pairs of SSR primers and 48 pairs of AFLP primer, 341 and 1119 bands were amplified, respectively. Among these 1460 bands, 557 bands (39.52%) were polymorphic, including 184 bands by SSR markers and 393 bands by AFLP markers. In the group of these polymorphic bands, 84 bands from SSR markers and 108 bands from AFLP markers showed the genetic distortion (P = 0.05). A total of 192 segregation distortion markers were obtained in this study. By using the JoinMap 4.0 software to do the linkage analysis, a genetic linkage map was established with length of 2759.4 cM, consisted of 17 linkage groups, and comprised of 495 polymorphic molecular markers including 170 segregation distortion markers. The mean marker interval distance is 5.57 cM between markers. In addition, the number of markers in the linkage groups varied from 5 to 72, and the length of linkage groups were from 68.88 cM to 250.17 cM. The genetic map developed in the present study could be used for QTL mapping and gene cloning of sunflower important genes.

Key words: sunflower, recombinant inbred lines population, SSR, AFLP, molecular markers linkage map

张永虎,于海峰,侯建华,李素萍,吕品,于志贤. 利用向日葵重组自交系构建遗传图谱[J]. 遗传, 2014, 36(10): 1036-1042.

Yonghu Zhang, Haifeng Yu, Jianhua Hou, Suping Li, Pin Lv, Zhixian Yu. Construction of a genetic map of sunflower using a population of recombinant inbred lines (RILs)[J]. HEREDITAS(Beijing), 2014, 36(10): 1036-1042.

参考文献

[1] 贺超英, 王永军, 张志永, 东方阳, 张劲松, 陈受宜, 盖钧镒. 大豆遗传图谱的构建和分析. 遗传学报, 2001, 28(11): 1051-1061.
[2] P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res , 1995, 23(21): 4407-4414.
[3] 丁毅. 基于AFLP标记的中国西藏近缘野生大麦遗传多样性分析. 遗传, 2007, 29(6): 725-730.
[4] L, Vear F, Zhang YX, Bervillé A, Nicolas P. Development of a consensus linkage RFLP map of cultivated sunflower ( Helianthus annuus L.). Theor Appl Genet , 1995, 90(7-8): 1079-1086.
[5] L, Mestries E, Mouzeyar S, Mouzeyar F, Badaoui S, Vear F, de Labrouhe D T, Nicolas P. A composite map of expressed sequences and phenotypic traits of the sunflower ( Helianthus annuus L.) genome. Theor Appl Genet , 99(1-2): 218-234.
[6] RP, Peleman J. The catisol sunflower RFLP map (146 loci) extended with 291 AFLP markers. In: Proceed of the 18th sunflower research workshop. Fargo , North Dakota, USA , , 1996: 174-178.
[7] S, Yu JK, Slabaugh MB, Shintani K, Knapp J. Simple sequence repeat map of the sunflower genome . Theor Appl Genet , 2002(8), 105: 1124-1136.
[8] Genzbitelle L, Fabre F, Saraffi A. SSR分子标记丰富向日葵( Helianthus annuus L.)遗传图谱的研究. 西南农业学报, 2012, 25(3): 1031-1038.
[9] Berrios E, Gentzbittel L, Kayyal H, Alibert G, Sarrafi A. AFLP mapping of QTLs for in vitro organogenesis traits using recombinant inbred lines in sunflower ( Helianthus annuus L.). Theor Appl Genet , 2000, 101(8): 1299-1306.
[10] 李方, 徐昌杰, 张上隆, 傅承新. 改良CTAB法用于多年生植物组织基因组DNA的大量提取. 遗传, 2004, 26(4): 529-531.
[11] 陈雪, 于海峰, 侯建华, 斯日古楞. 向日葵芽期抗旱相关性状丙二醛含量的SSR分子标记分析. 内蒙古农业大学学报(自然科学版), 2012, 33(3): 25-29.
[12] BJ, Caetano-Anolles G, Gresshoff PM. Fast and sensitive silver staining of DNA in polyacrylamide gels. Annal Bio Chem , 1991, 196(1): 80-83.
[13] SP, Lan TH, Feldmann KA, Paterson AH. Comparative mapping of Arabidopsis thaliana and Brassica oleracea chromosomes reveals islands of conserved organization. Genetics , 1994, 138(2): 499-510.
[14] 傅骏骅, Warburton M, 李新梅, 张世煌, Khairallah M, 刘新芝, 彭泽斌, 李连城. 利用RFLP、SSR、AFLP和RAPD标记分析玉米自交系遗传多样性的比较研究. 遗传学报, 2000, 27(8): 725-733.
[15] SX, Kishore VK, Knapp SJ. PCR-multiplexes for a genome-wide framework of simple sequence repeat marker loci in cultivated sunflower. Theor Appl Genet , 2003, 107(1): 6-19.
[16] 张宗文, 张恩来, 吴斌. 大粒裸燕麦( Avena nuda L.)遗传连锁图谱的构建. 植物遗传资源学报, 2013, 14(4): 673-678
[17] 安玉麟, 孙瑞芬, 张艳芳. 向日葵rRNA基因克隆及其染色体定位研究. 中国细胞生物学学报, 2014, 36(2): 195-199.
[18] W, Thomas WTB, Baird E, Lawrence P, Booth A, Harrower B, Mcnicol JW, Waugh R. Analysis of quantitative traits in barley by the use of Amplified Fragment Length Polymorphisms. Heredity , 1997, 79(1): 48-59.
[19] 刘公社, 齐冬梅, 汪恩华, 李芳芳. 向日葵品种鉴定和纯度分析的AFLP研究. 植物学通报, 2002, 19(4): 444-451
[20] 王永健, 郑晓鹰. 大白菜分子遗传图谱的构建与分析. 中国农业科学, 2003, 36(2): 190-195.
[21] T, Yano Y, Abe K, Geographic distribution of alleles at the Gal2 locus for segregation distortion in barley. Theor Appl Genet , 1992, 85(4): 419-422.
[22] MR, Ellis TH. Excess heterozygosity contributes to genetic map expansion in pea recombinant inbred populations. Genetics , 2002, 162(2): 861-873.
[23] 崔金腾, 高用明. 遗传群体偏分离研究进展. 植物遗传资源学报, 2009, 10(4): 613-617.
[24] 萝卜遗传连锁图谱构建与主要品质性状 QTLs 分析. QTLs分析[学位论文]. 南京: 南京农业大学, 2009: 34-35.
[25] 陈德富)

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

利用向日葵重组自交系构建遗传图谱

Construction of a genetic map of sunflower using a population of recombinant inbred lines (RILs)

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	孙高飞,何守朴,潘兆娥,杜雄明. 棉属四倍体AD₁与二倍体A₂、D₅基因组的同源SSR分析[J]. 遗传, 2015, 37(2): 192-203.
[2]	赵巧玲, 王文波, 陈安利, 裘智勇, 夏定国, 钱荷英, 沈兴家. 家蚕两种新的类鹑斑突变体的遗传分析和SSR标记定位[J]. 遗传, 2014, 36(4): 369-375.
[3]	任勇, 李生荣, 罗建明, 何中虎, 杜小英, 周强,何员江,魏育明, 郑有良. 绵麦37特异位点在其衍生品种中的遗传贡献率分析[J]. 遗传, 2014, 36(2): 145-151.
[4]	李峰利, 狄佳春, 赵亮, 陈旭升. 陆地棉皱缩叶突变体基因wr3的初步定位[J]. 遗传, 2014, 36(12): 1256-1260.
[5]	温莹逯晓萍任锐米福贵韩平安薛春雷. 高丹草EST-SSR标记的开发及其遗传多样性[J]. 遗传, 2013, 35(2): 225-232.
[6]	严红梅，董超，张恩来，汤翠凤，阿新祥，杨文毅，杨雅云，张斐斐，徐福荣. 微卫星标记分析水稻地方品种30年的遗传变异[J]. 遗传, 2012, 34(1): 87-94.
[7]	王遵宝，赵宗胜，余鹏，吴洪宾，班谦，梁耀伟，郑炜. 绵羊皮肤源EST-SSR标记的功能注释及染色体电子定位[J]. 遗传, 2011, 33(7): 731-737.
[8]	彭文舫，吕建伟，任小平，黄莉，赵新燕，文奇根，姜慧芳. 花生抗青枯病相关基因的差异表达[J]. 遗传, 2011, 33(4): 389-396.
[9]	景超，马晓杰，狄佳春，陈旭升. 陆地棉超矮杆突变体基因的初步定位[J]. 遗传, 2011, 33(12): 1393-1397.
[10]	冯素萍，李维国，于飞，王静毅，武耀廷. 巴西橡胶树SSR遗传图谱的构建[J]. 遗传, 2010, 32(8): 857-863.
[11]	马晋，李涛，龙文敏，安玮玮，郭亚平，马恩波. 中华稻蝗不同地理种群遗传多样性的AFLP分析[J]. 遗传, 2010, 32(2): 163-169.
[12]	朱其卫，李火根. 鹅掌楸不同交配组合子代遗传多样性分析[J]. 遗传, 2010, 32(2): 183-188.
[13]	轩楠，牛宝龙，王海龙，庄俐，孟智启. 家蚕性连锁平衡致死系致死基因的SSR定位[J]. 遗传, 2010, 32(12): 1269-1274.
[14]	王修业，李木旺，赵云坡，徐安英，郭秋红，黄勇平，郭锡杰. 结合SSR标记和STS标记对家蚕无鳞毛翅基因的定位[J]. 遗传, 2010, 32(1): 54-58.
[15]	晏慧君，张颢，谢吉容，李树发，蹇洪英，邱显钦，王其刚，王继华，唐开学. 基于差减cDNA文库EST信息的月季花香突变体SSR标记的开发[J]. 遗传, 2009, 31(9): 962-966.