巴西橡胶树SSR遗传图谱的构建

doi:10.3724/SP.J.1005.2010.00857

遗传 ›› 2010, Vol. 32 ›› Issue (8): 857-863.doi: 10.3724/SP.J.1005.2010.00857

巴西橡胶树SSR遗传图谱的构建

冯素萍^{1, 3},李维国²,于飞^{1, 4},王静毅^{1, 4},武耀廷³

1. 中国热带农业科学院热带作物生物技术研究所, 海口 571101; 2. 中国热带农业科学院橡胶研究所, 儋州 571737; 3. 琼州学院, 五指山 572200; 4. 海南大学农学院,海口 570228

收稿日期:2009-10-16 修回日期:2010-01-05 出版日期:2010-08-20 发布日期:2010-08-23
通讯作者: 武耀廷 E-mail:wuyaoting@tsinghua.org.cn
基金资助:
中央级公益性科研院所基本科研业务费项目(编号：ITBBZD0715)资助

Construction of genetic linkage map for rubber tree (Hevea brasiliensis)based on SSR markers

FENG Su-Ping^{1, 3}, LI Wei-Guo², YU Fei^{1, 4}, WANG Jing-Yi^{1, 4}, WU Yao-Ting³

1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China; 2. Rubber Cultivation Research Institute, Chinese Academy of Tropic Agricultural Science, Danzhou 571737, China; 3. Qiongzhou University, Wuzhishan 572200, China; 4. Agricultural College, Hainan University, Haikou 570228, China

Received:2009-10-16 Revised:2010-01-05 Online:2010-08-20 Published:2010-08-23
Contact: WU Yao-Ting E-mail:wuyaoting@tsinghua.org.cn

摘要/Abstract

摘要： 以热研88-13×IAN873的94个F1群体为试材, 利用简单序列重复(Simple sequence repeat, SSR)标记, 采用FsLinkageMAP 1.0软件, 构建了巴西橡胶树热研88-13×IAN873的遗传连锁图谱。从441对SSR引物中筛选出160对具有多态信息的引物, 在分离群体中共检测到206个多态性位点, 176个位点用于遗传图谱的构建; χ²检验结果显示, 有147个位点符合1:1分离比例, 有12个符合1:2:1分离比例, 有17个符合1:1:1:1的分离比例, 共有13个偏分离位点, 偏分离率低(7.38%); 91个SSR位点被分为18个连锁群, 覆盖橡胶树基因组1 937.06 cM, 每个连锁群包含2～16个位点, 标记间的平均距离为21.29 cM。

关键词: 巴西橡胶树, SSR标记, 遗传图谱

Abstract: Out of 260 polymorphic loci screened from a total of 441 pairs of EST-SSR and genomic-SSR primers, 176 were used for constructing the genetic map of rubber tree (Hevea brasiliensis) by an F1 segregating population including 94 progenies from the cross Reyan 88-13×IAN873. Chi-square test carried out on the polymorphic loci used in constructing the map showed that 147 loci followed a segregation ratio of 1:1 and 12 loci followed a ratio of 1:2:1 and 17 loci followed a ratio of 1:1:1:1. Only 13 (7.38%) loci were distorted from the Mendelian ratio. The genetic linkage map consisted of 91 marker loci in 18 linkage groups and covered 1 937.06 cM with an average genetic distance of 21.29 cM between adjacent markers. The largest linkage group consisted of 16 marker loci, while the smallest one contained only 2 marker loci.

Key words: rubber tree (Hevea brasiliensis), SSR markers, genetic lingkage map

冯素萍，李维国，于飞，王静毅，武耀廷. 巴西橡胶树SSR遗传图谱的构建[J]. 遗传, 2010, 32(8): 857-863.

FENG Su-Ping, LI Wei-Guo, XU Fei, WANG Jing-Yi, WU Yao-Ting. Construction of genetic linkage map for rubber tree (Hevea brasiliensis)based on SSR markers[J]. HEREDITAS, 2010, 32(8): 857-863.

参考文献

[1] Paglia GP, Olivieri AM, Morgante M. Towards sec-ond-generation STS (sequence-tagged sites) linkage maps in conifers: a genetic map of Norway spruce (Picea abies K.). Mol Gen Genet, 1998, 258(5): 466–478. [2] 安泽伟, 赵彥宏, 程汉, 李维国, 黄华孙. 橡胶树EST-SSR标记的开发和应用. 遗传, 2009, 31(3): 311-319. [3] Brown GR, Kadel III EE, Bassoni DL, Kiehne KL, Te-mesgen B, van Buijtenen JP, Swell MM, Marshall KA, Neal DB. Anchored reference loci in loblolly pine (Inus teada L.) for integrating pine genomics. Genetics, 2001, 159(2): 799–809. [4] Lespinasse D, Rodier-Goud M, Grivet L, Leconte A, Leg-nate H, Seguin M. A saturated genetic linkage map of rubber tree (Hevea spp.) based on RFLP, AFLP, microsa-tellite, and isozyme markers. Theor Appl Genet, 2000, 100: 127–138. [5] Venkatachalam P, Priya , Amma CK, Thulaseedharan A. Identification, cloning and sequencing analysis of a dwarf genomic-specific RAPD marker in rubber tree [Hevea brasiliensis (Muell.) Arg.]. Plant Cell Rep, 2004, 23(5): 327–332. [6] 陈守才, 邵寒霜, 胡东琼. 用RAPD技术鉴定橡胶树抗白粉病基因连锁标记. 热带作物学报, 1994, 15(2): 21–26. [7] Le Guen V, Lespinasse D, Oliver G, Rodier-Groud M, Pi-nard F, Seguin M. Molecular mapping of genes conferring field resistance to South American Leaf Blight (Microcyclus ulei) in rubber tree. Theor Appl Genet, 2003, 108(1): 160–167. [8] 和丽岗. 橡胶树分子遗传图谱的构建及相关性状的QTL初步定位[学位论文]. 华南热带农业大学, 2007. [9] 王惠君. 橡胶树初级分子遗传图谱的构建[学位论文]. 华南热带农业大学, 2007. [10] Venkatachalam P, Thomas S, Priya P, Thanseem I, Gireesh T,Saraswathyamma CK, Thulaseedharan A. Identification of DNA polymorphism within the clones of Hevea brasiliensis (Muell.) Arg. using RAPD analysis. Indian J Nat Rubb Res, 2002, 15(3): 172–181. [11] Feng SP, Li WG, Huang HS, Wang JY, Wu YT. Develop-ment, application and cross-species/genera transferability of EST-SSR makers for rubber tree (Hevea brasiliensis). Mol Breed, 2009, 23: 85–97. [12] 张军, 张天真. 棉花微卫星标记的PAGE/银染快速检测. 棉花学报, 2000, 12(5): 267–269. [13] 施季森, 童春发. 树木遗传图谱构建和QTL定位统计分析. 北京: 科学出版社 2006, 82–86. [14] Hemma TM, Weedon NF, Manganaris, AG, Lawson DM. Molecular marker linkage map for apple. J Hered, 1994, 85(1): 4–11. [15] Testolin R, Huang WG, Lain O, Messina R, Vecchione A, Cipriani G. A kiwifruit (Actinidia spp.) linkage map based on microsatellites and integrated with AFLP markers. Theor Appl Genet, 2001, 103: 30–36. [16] Porceddu A, Albertini E, Barcaccia G, Falistocco E, Fal-cinelli M. Linkage mapping in apomictie and sexual Ken-tucky bluegrass (Poa pratensis L.) genotypes using a two-way pseudo-testcross strategy based on AFLP and SAMPL markers. Theor Appl Genet, 2002, 104(2-3): 273–280. [17] 张德强, 张志毅, 宋婉等. 毛白杨遗传作图最适分离群体的选择. 北京林业大学学报, 2003, 25(4): 21–25. [18] 窦美安, 郭森元, 叶应福. 中规模推广级橡胶抗寒高产品种IAN873的引种利用研究. 热带作物学报, 2002, 23(3): 21–26. [19] 黄华孙. 中国橡胶树育种五十年. 北京: 中国农业出版社, 2005, 170–190. [20] 高妍, 韩明玉, 赵彩平, 宋健. 桃分子连锁图谱的构建. 果树学报, 2008, 25(4): 478–484. [21] Temesgen B, Brown GR, Harry DE, Kinlaw CS, Sewell MM, Neale DB. Genetic mapping of expressed sequence tag polymorphism (ESTP) markers in loblolly pine (Pinus taeda L.). Theor Appl Genet, 2001, 102(5): 664–675. [22] Nikaido AM, Ujino T, Iwata H, Yoshimura K, Yoshimura H, Suyama Y, Murai M, Nagasaka K, Tsumura Y. AFLP and CAPS linkage maps of Cryptomeria japonica. Theor Appl Genet, 2000, 100(6): 825–831. [23] Xu SJ, Singh RJ, Hymowitz T. Establishment of a cyto-genetic map soybean: progress and prospective. Soybean Genet Newslett, 1997, 24: 121–122. [24] Kinishita T. Report of the committee on gene mbolization, nomenclature and linkage group. Rice Genet Newslett, 1991, 8: 2–37. [25] Tzeng TH, Lyngholm LK, Ford CF, Bronson CR. A re-striction fragment length polymorphism map and electro-

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

巴西橡胶树SSR遗传图谱的构建

Construction of genetic linkage map for rubber tree (Hevea brasiliensis)based on SSR markers

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	任勇, 李生荣, 罗建明, 何中虎, 杜小英, 周强,何员江,魏育明, 郑有良. 绵麦37特异位点在其衍生品种中的遗传贡献率分析[J]. 遗传, 2014, 36(2): 145-151.
[2]	王洋坤, 胡艳, 张天真. RAD-seq技术在基因组研究中的现状及展望[J]. 遗传, 2014, 36(1): 41-49.
[3]	严红梅，董超，张恩来，汤翠凤，阿新祥，杨文毅，杨雅云，张斐斐，徐福荣. 微卫星标记分析水稻地方品种30年的遗传变异[J]. 遗传, 2012, 34(1): 87-94.
[4]	王贤磊，高兴旺，李冠，王惠林，耿守东，康锋，聂祥祥. 甜瓜遗传图谱的构建及果实与种子QTL分析[J]. 遗传, 2011, 33(12): 1398-1408.
[5]	王峰，官春云. 甘蓝型油菜遗传图谱的构建及单株产量构成因素的QTL分析[J]. 遗传, 2010, 32(3): 271-277.
[6]	黄关青，华玉伟，高战，黄华孙. 橡胶树甲基化过滤文库的构建[J]. 遗传, 2010, 32(10): 1071-1076.
[7]	赵向前，吴为人. 水稻ILP标记遗传图谱的构建[J]. 遗传, 2008, 30(2): 225-230.
[8]	梁永书，彭勇，叶少平，李平，孙林静，马忠友，李艳萍. 水稻籼粳交F2、F6群体遗传连锁图谱的比较分析[J]. 遗传, 2007, 29(9): 1110-1120.
[9]	张所兵，朱镇，赵凌，张亚东，陈涛，林静，王才林. 水稻长穗颈基因eui紧密连锁SSR标记获得[J]. 遗传, 2007, 29(3): 365-365―370.
[10]	金基强，崔海瑞，龚晓春，陈文岳，忻雅. 用EST-SSR标记对茶树种质资源的研究[J]. 遗传, 2007, 29(1): 103-103―108.
[11]	陈永华，赵森，柳俊，严钦泉，肖国樱. 水稻耐淹涝性状的遗传分析和SSR标记的研究 [J]. 遗传, 2006, 28(12): 1562-1562~1566.
[12]	杨本超，肖炳光，陈学军，石春海. 基于ISSR标记的烤烟种质遗传多样性研究[J]. 遗传, 2005, 27(5): 753-758.
[13]	李明芳，郑学勤. 荔枝SSR标记的研究[J]. 遗传, 2004, 26(6): 911-916.
[14]	李明芳，郑学勤. 开发SSR引物方法之研究动态[J]. 遗传, 2004, 26(5): 769-776.
[15]	王宏林，喻德跃，王永军，陈受宜，盖钧镒. 应用重组自交系群体定位大豆根重QTL[J]. 遗传, 2004, 26(3): 0-329.