遗传 ›› 2008, Vol. 30 ›› Issue (2): 225-230.doi: 10.3724/SP.J.1005.2008.00225

• 研究报告 • 上一篇    下一篇

水稻ILP标记遗传图谱的构建

赵向前1, 2, 吴为人1   

  1. 1. 浙江大学农业与生物技术学院, 杭州 310029;
    2. 浙江省嘉兴市农业科学研究院, 嘉兴 314016

  • 收稿日期:2007-08-22 修回日期:2007-10-25 出版日期:2008-02-10 发布日期:2008-02-10
  • 通讯作者: 吴为人

Construction of a genetic map based on ILP markers in rice

ZHAO Xiang-Qian1,2, WU Wei-Ren1

  

  1. 1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; 2. Jiaxing Academy of Agricultural Science, Jiaxing 314016, China
  • Received:2007-08-22 Revised:2007-10-25 Online:2008-02-10 Published:2008-02-10
  • Contact: Wu Wei-Ren

摘要:

内含子长度多态性(ILP)是一种基于PCR的新型分子标记, 具有许多突出的优点。我们先前利用已公布的籼稻品种93-11和粳稻品种日本晴的基因组序列数据, 已开发了172个水稻ILP标记。为了检验这些ILP标记的可靠性及其在遗传作图中的可用性, 利用一个BC1F1(日本晴/93-11//日本晴)群体, 构建了一张含172个ILP标记座位和13个SSR标记座位的水稻遗传图谱, 总长度为1 905.7 cM。比较显示, 图谱上所有标记的顺序与其物理顺序完全一致, 证明了利用ILP标记进行遗传作图的可行性和有效性。文中还对标记偏分离现象进行了分析, 发现在第6号染色体短臂上存在一个严重偏分离的区域。

关键词: 遗传图谱, ILP标记, 水稻

Abstract:

Intron length polymorphism (ILP) is a new type of PCR-based molecular markers with many advantages. We had previously developed 172 ILP markers in rice using the published genome sequence data of indica cultivar 93-11 and ja-ponica cultivar Nipponbare. In order to examine the reliability and the applicability of these ILP markers to genetic map-ping, we constructed a rice genetic map consisting of 172 ILP and 13 SSR markers with a total length of 1 905.7 cM using a BC1F1 (Nipponbare/93-11//Nipponbare) population. Comparison showed that the order of the markers in this map was ex-actly the same as their physical order, verifying the feasibility and efficiency of using ILP markers for genetic mapping. We also investigated the phenomenon of marker segregation distortion and found a region of serious segregation distortion in the short arm of chromosome 6.