拟南芥AtWRKY25、AtWRKY26和AtWRKY33在非生物胁迫条件下的表达分析

doi:10.3724/SP.J.1005.2010.00848

遗传 ›› 2010, Vol. 32 ›› Issue (8): 848-856.doi: 10.3724/SP.J.1005.2010.00848

拟南芥AtWRKY25、AtWRKY26和AtWRKY33在非生物胁迫条件下的表达分析

付乾堂^{1, 2}, 余迪求¹

1. 中国科学院西双版纳热带植物园, 昆明 650223; 2. 中国科学院研究生院, 北京 100049

收稿日期:2009-10-28 修回日期:2010-01-14 出版日期:2010-08-20 发布日期:2010-08-23
通讯作者: 余迪求 E-mail:ydq@xtbg.ac.cn
基金资助:
转基因生物新品种培育科技重大专项(编号：2009ZX08009-066B)和国家自然科学基金项目(编号：90817003)资助

Expression profiles of AtWRKY25, AtWRKY26 and AtWRKY33 under abiotic stresses

FU Qian-Tang^{1, 2}, YU Di-Qiu¹

1. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China; 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2009-10-28 Revised:2010-01-14 Online:2010-08-20 Published:2010-08-23
Contact: YU Di-Qiu E-mail:ydq@xtbg.ac.cn

摘要/Abstract

摘要： WRKY 转录因子家族在调控植物逆境诱导反应、生长发育及其信号转导等方面起着重要的分子生物学功能。文章采用Northern 杂交的方法, 对拟南芥3个WRKY基因进行表达谱分析。结果表明: AtWRKY25、AtWRKY26和AtWRKY33受多种非生物逆境因子(温度因子、高盐、渗透胁迫和激素脱落酸)的影响, 其中低温和高盐对AtWRKY25、AtWRKY26和AtWRKY33的诱导尤为明显, 表明这3个AtWRKY基因可能在响应环境信号方面起着一定的作用。作为序列相似性较高的AtWRKY25、AtWRKY26和AtWRKY33对一些胁迫因子的表达模式呈现一定的相似性; 但AtWRKY33受高温的抑制和低温的快速诱导, 与另外两个基因的表达模式不同, 推测它们对温度胁迫因子的反应存在差异。此外, 对启动子序列的生物信息学分析发现, 3个基因的启动子包含多个与非生物逆境反应相关的顺式作用元件。

关键词: 拟南芥, AtWRKY基因, 非生物胁迫, 表达谱

Abstract: The transcription factor WRKY family is one type of key regulatory components of plant development and defense against stress factors. The expression profiles of three AtWRKY genes under abiotic stresses were analyzed by Northern blotting analysis. The expression of AtWRKY25, AtWRKY26, and AtWRKY33 changed during stress treatments including thermal factors, NaCl, abscisic acid (ABA) and osmotic stress, and significantly under NaCl and cold treatments, suggesting a specific role of the three AtWRKYs in adaptation to environmental stresses in plants. We also found that the three AtWRKY genes showed distinct expression patterns under thermal stresses. AtWRKY25 and AtWRKY26 were gradually induced during heat and cold treatments, whereas AtWRKY33 was suppressed by heat treatment and induced rapidly during cold stress, indicating that the three AtWRKYs may play different roles in response to temperature factors. In addition, we analyzed the sequence of the promoters with bioinformatics approach, and some cis-elements involved in abiotic stresses and hormonal responses were revealed. The results provided important information for studying biological functions of three AtWRKY genes.

Key words: Arabidopsis thaliana, AtWRKY geneIabiotic stressesIexpression profiles

付乾堂，余迪求. 拟南芥AtWRKY25、AtWRKY26和AtWRKY33在非生物胁迫条件下的表达分析[J]. 遗传, 2010, 32(8): 848-856.

FU Gan-Tang, TU Di-Qiu. Expression profiles of AtWRKY25, AtWRKY26 and AtWRKY33 under abiotic stresses[J]. HEREDITAS, 2010, 32(8): 848-856.

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拟南芥AtWRKY25、AtWRKY26和AtWRKY33在非生物胁迫条件下的表达分析

Expression profiles of AtWRKY25, AtWRKY26 and AtWRKY33 under abiotic stresses

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