遗传 ›› 1997, Vol. 19 ›› Issue (3): 5-8.
宋苏祥1; 刘洪柏1; 孙大江1; 范兆廷1; 桂建芳2 SONG Su-Xiang1;LIU Hong-Bai1;SUN Da-Jiang1;FAN Zhao-Ting1;GUI Jian-Fang
摘要: 采用体内注射小牛血清、肾组织细胞短期培养、常见空气干燥法制备了施氏鲟(Acipenser schrencki Brandt)的染色体,并进行了核型分析。施氏鲟二倍体的染色体为238±8条,其核型为78m+12sm+28st,t+120±mc,NF :328±。以外周血红细胞为样本,鸡的红细胞为对照,用美国产的FACStar Plus流式细胞仪测定了施氏鲟二倍体细胞核的DNA含量, 其DNA含量为鸡的5.06倍,绝对含量为11.73±0.68pg/N。
Abstract:Metaphase chromosome specimens of Amur Sturgeon,injected with fetalcalf serum,were prepared from short-term culture of kidney cells with air-drying technique.Its diploid chromosome number is 2n=238±8.Karyotype consists of 78m+12sm+28st,t+120±mc,NF:328±.Diploid nucleus DNA content was measured from the peripheral erythrocytes,using flow cytometer(FACStar Pus,made in USA)and erythrocytes of chick(Gallus sp)as internal standard.DNA content of the fish is 11.73±0.68pg/N and the ratio to that of chickens is 5.06.