遗传 ›› 1998, Vol. 20 ›› Issue (4): 23-25.

• 论文 • 上一篇    下一篇

条纹斑竹鲨线粒体DNA的研究

吴冰; 陈元霖; 桂慕燕 WU Bing;CHEN Yuan-lin;GUI Mu-yan   

  1. 福建省厦门大学细胞生物学研究室,厦门 361005 Laboratory of Cell Biology,Xiamen University of Fujan Province,Xiamen 361005
  • 收稿日期:1900-01-01 出版日期:1998-08-10 发布日期:1998-08-10

Studies on Mitochondrial DNA of Chiloscyllium plagiosum

  • Received:1900-01-01 Online:1998-08-10 Published:1998-08-10

摘要: 用6种限制性内切酶分析了4条条纹斑竹鲨的线粒体DNA(mtDNA)。PstⅠ、Hpa
Ⅰ、XbaⅠ、EcoRⅠ、EcoRⅤ、BglⅡ在条纹斑竹鲨mtDNA分子上分别具有0至2个切点,
mtDNA分子大小为16.6kb,根据单酶和双酶完全酶解片段的大小,构建了条纹斑竹鲨mtDNA
的限制性酶切图谱。
Abstract:Mitochondrial DNA(mtDNA)form 4 samples of Chiloscyllium plagiosum was analyzed by 6 kinds of restriction.The number of cleavage sites were as follow:2 for HpaI,XbaI and EcoRI respectively;1 for BglII and EcoRV respectively;None for PstI.Molecular size of mtDNA was found to be 16.6kb.According to analysis of single and double enzyme cleavage,the map of restriction enzyme was constructed.

关键词: 限制性酶切图谱
Key words,
Chiloscyllium plagiosum, mtDNA, 线粒体DNA, 条纹斑竹鲨, 种系鉴定, 线粒体DNA, 鲫鱼

Key words: Stock identification, Carassius aurantus, mtDNA