遗传 ›› 2001, Vol. 23 ›› Issue (4): 306-308.

• 论文 • 上一篇    下一篇

特异性PCR反应鉴别携带W染色体的嵌合体公鸡子代性别的研究

燕海峰1;P.Trefil2;肖兵南1;吴晓林1 YAN Hai-fengI;P.Trefil2;XIAO Bing-nanI;WU Xiao-lin1   

  1. 1。湖南省畜牧兽医研究所,长沙 410131 2.Research Institute of Biopharmacy and Veterinary Drugs,25449 Jilove,Prague,Czech Republic 1.Hunan Institute of Animal and Veterinary Science,Changsha 410131,China; 2.Research Institute of Biopharmacy and Veterinary Drugs,25449 Jilove,Prague,Czech Republic
  • 收稿日期:1900-01-01 出版日期:2001-08-10 发布日期:2001-08-10

Sex Determination of Offspring of the Chimeric Cocks Carrying a W Chromosome Based on Specific PCR

  • Received:1900-01-01 Online:2001-08-10 Published:2001-08-10

摘要: 利用聚合酶链式反应(PCR)特异扩增家鸡W染色体高度重复序列EcoRI家族的447bp片段,作为鉴定家鸡W染色体存在的依据.4个表现型为雄性的嵌合体公鸡的精液中检测到了W染色体,通过人工授精获得嵌合体子一代.检测了其中188个雏鸡的血液,51.6%(97/188)含有W染色体,x2检验结果显示,雌雄后代符合1:1的比例,这表明在这188个成活的嵌合体子代中,基本上没有W染色体精子所产生的雏鸡,携带W染色体的精子没有将其W染色体传递到其子代雏鸡中.本研究利用PGR性别鉴定技术,从分子水平上对精液中携带W染色体的嵌合体公鸡(ZW→ZZ型)子代的性别分化作了初步的探讨。
Abstract:The W chromosome was detected in four cocks' sperms by a method based on the polymerase chain reaction which amplifies the EcoRI repeat unit of the chicken W chromosome.188 offsprings of these chimeric cocks were obtained by artificial insemination and 51.6% (97/188) was detected W chromosome in blood.The result of χ2 test suggests that the sex ratio is 1:1.It seems as if no evidence was found for skewed sex ratios of these offsprings.Therefore it seems as if the sperms with W chromosome could not contribute to these 188 offspring chicks.

关键词: 嵌合体鸡, W染色体, PCR, 性别鉴定
Key words,
PCR