摘要： 用6种限制性内切酶对洱海的四种鲤鱼-洱海鲤(C.barbatus)、春鲤(C.longipectoralis)、大眼鲤(C.megalophthalmus)和杞麓鲤(C.carpio chila),其中前三种为洱海特有,进行了线粒体DNA(mtDNA)的限制性片段长度多态(RFLP)分析,构建了它们的mtDNA限制性内切酶图谱.结果表明,这四种鲤鱼在种内和种间均缺乏mtDNA RFLP.这种现象在鱼类种间的mtDNA的RFLP研究中是罕见的.分析这一现象的原因,可能在于这些物种是同域形成物种,并且其分化时间还相当短。
Abstract:Restriction fragment length polymorphism(RFLP)analysis of mitochondrial DNA(mtDNA)showed that there is no genetic divergence both intra-and interspecific of 4 species of Cyprinus(C.barbatus,C.longipectoralis,C.megalophthalmus and C.carpio chila)in Erhai lake,Yunnan.Lack of interspecific mtDNA RFLP,namely the close genetic similarity of these four fish species,is possibly resulted from the sympatric speciation and their short divergence time.In addition,the restriction map was made by double digestion.