猪ATF4基因多态性与生产性状的关联及基因表达分析

doi:10.3724/SP.J.1005.2011.01347

遗传 ›› 2011, Vol. 33 ›› Issue (12): 1347-1352.doi: 10.3724/SP.J.1005.2011.01347

猪ATF4基因多态性与生产性状的关联及基因表达分析

陈超, 吴望军, 熊远著

华中农业大学农业部猪遗传育种重点开放实验室, 武汉 430070

收稿日期:2011-01-20 修回日期:2011-03-30 出版日期:2011-12-20 发布日期:2011-12-25
通讯作者: 熊远著 E-mail:xiongyzh@mail.hzau.edu.cn
基金资助:
国家重点基础研究发展规划(973 计划)项目(编号：2006CB102102)资助

Expression analysis of porcine ATF4 gene and its association with production traits

CHEN Chao, WU Wang-Jun, XIONG Yuan-Zhu

Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China

Received:2011-01-20 Revised:2011-03-30 Online:2011-12-20 Published:2011-12-25

摘要/Abstract

摘要： 为进一步了解和认识ATF4基因的功能, 揭示ATF4对猪脂肪代谢的影响, 寻找与肉质性状相关联的分子标记, 文章采用PCR方法扩增了ATF4基因部分序列, 通过序列比对发现在翻译起始密码子ATG下游159 bp处存在A159G转换, 通过PCR-AluⅠ-RFLP对大白猪、长白猪、梅山猪和通城猪进行酶切分型, 发现在大白猪和长白猪中均为AA基因型, 在梅山猪和通城猪中均为GG基因型。进一步对大白猪×梅山F₂群体资源家系进行了酶切分型, 并分析该位点的多态性与生产性状的关系。结果表明, ATF4的多态性与臀部平均膘厚存在极显著相关(P<0.01), 与胸腰椎间膘厚、平均膘厚、眼肌高、眼肌面积存在显著相关(P<0.05)。采用Real-time PCR分析了ATF4基因在大白猪与梅山猪背最长肌不同发育阶段的表达模式。结果表明, ATF4基因在大白猪和梅山猪胚胎期65 d和出生后3 d中的表达水平相对都比较低, 且在两品种间无明显差异; 而在出生后60 d和120 d, ATF4基因在大白猪中与梅山猪均出现了上调表达, 并且在梅山猪中的相对表达水平要显著高于大白猪。研究结果为进一步深入研究猪ATF4基因在脂肪代谢中的分子机理奠定了基础。

关键词: 猪, ATF4, PCR-RFLP, 关联分析, 表达模式分析

Abstract: In order to understand the function of gene ATF4 and identify new DNA markers involved in pig production traits, the cDNA fragment of porcine ATF4 was cloned and sequenced. Sequence comparison revealed an A159G substitu-tion downstream of the initiation codon (ATG). We then carried out PCR-AluⅠ-RFLP analysis in Large white, Landrace, Tongcheng and Meishan pigs, followed by association analysis in F₂ “Large white ×Meishan” resource family. In all the individuals tested, Large White and Landrace pigs possessed the AA genotype, while Meishan and Tongcheng pigs possessed the GG genotype. Association analysis in F₂ resource family showed that this site was highly associated with buttock fat thickness (BFT) (P<0.01) and had significant effect on thorax-waist fat thickness (TFT), average backfat thickness (ABT), loin eye height (LEH), and loin eye area (LEA)(P<0.05). Real-time PCR was used to analyze the expression patterns of porcine ATF4 gene in longissimus dorsi at different development stages of Large White and Meishan pigs. The results showed that the gene expression levels of ATF4 were low 65 days after conception and 3 days after birth, but no significant differences were observed in both breeds. Meanwhile, the expression levels of porcine ATF4 gene were up-regulated 60 days and 120 days after birth in both breeds and the expression level in Meishan pigs was obviously higher than that in Large White pigs. These data could lay the foundation for further study on the molecular mechanism of porcine ATF4 gene in lipid metabolism.

Key words: porcine, ATF4, PCR-RFLP, association analysis, expression pattern analysis

陈超，吴望军，熊远著. 猪ATF4基因多态性与生产性状的关联及基因表达分析[J]. 遗传, 2011, 33(12): 1347-1352.

参考文献

[1] Fernandez X, Monin G, Talmant A, Mourot J, Lebret B. Influence of intramuscular fat content on the quality of pig meat-1. Composition of the lipid fraction and sensory characteristics of m. longissimus lumborum. Meat Sci, 1999, 53(1): 59-65.
[2] 姜延志, 刘晓研, 李芳琼, 李学伟. 猪H-FABP基因多态性及其与肌内脂肪含量的相关研究. 畜牧兽医学报, 2010, 41(7): 792-796.
[3] Siu F, Bain PJ, LeBlanc-Chaffin R, Chen H, Kilberg MS. ATF4 is a mediator of the nutrient-sensing response path-way that activates the human asparagine synthetase gene. J Biol Chem, 2002, 277(27): 24120-24127.
[4] Ye J, Koumenis C. ATF4, an ER stress and hypoxia-inducible transcription factor and its potential role in hy-poxia tolerance and tumorigenesis. Curr Mol Med, 2009, 9(4): 411-416.
[5] Wang WG, Lian N, Li LZ, Moss HE, Wang WX, Perrien DS, Elefteriou F, Yang XL. Atf4 regulates chondrocyte proliferation and differentiation during endochondral ossi-fication by activating Ihh transcription. Development, 2009, 136(24): 4143-4153.
[6] Seo J, Fortuno ES 3rd, Suh JM, Stenesen D, Tang W, Parks EJ, Adams CM, Townes T, Graff JM. Atf4 regulates obesity, glucose homeostasis, and energy expenditure. Diabetes, 2009, 58(1): 2565-2573.
[7] Wang CX, Huang ZY, Du Y, Cheng Y, Chen SH, Guo FF. ATF4 regulates lipid metabolism and thermogenesis. Cell Res, 2010, 20(2): 174-184.
[8] 熊远著, 邓昌彦. 种猪测定原理与方法. 北京: 中国农业出版社, 1999: 57-118.
[9] Liu BH. Statistical genomics: linkage, mapping, and QTL analysis. CRC Press, 1998.
[10] 黄京书, 熊远著. 猪ACTA2 基因的克隆、表达分析及其与生产性状的关联. 遗传, 2009, 31(5): 489-494.
[11] Livak KJ, Schmittgen TD. Analysis of relative gene ex-pression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 2001, 25(4): 402-408.
[12] 祁艳梅. 猪CREB家族基因的克隆及表达研究的初步探讨
[学位论文]. 武汉: 华中农业大学, 2009.
[13] Gerbens F, de Koning DJ, Harders FL, Meuwissen TH, Janss LL, Groenen MA, Veerkamp JH, Van Arendonk JA, te Pas MF. The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs. J Animal Sci, 2000, 78(3): 552-559.
[14] 王刚, 曾勇庆, 武英, 魏述东, 包新见, 刘婵娟, 孙延晓. 猪肌肉组织LPL基因表达的发育性变化及其与肌内脂肪沉积关系的研究. 畜牧兽医学报, 2007, 38(3): 253-257.
[15] Brun RP, Tontonoz P, Forman BM, Ellis R, Chen J, Evans RM, Spiegelman BM. Differential activation of adipo-genesis by multiple PPAR isoforms. Genes Dev, 1996, 10(8): 974-984.
[16] 柳晓峰, 李辉. PPAR基因与脂肪代谢调控. 遗传, 2006, 28(2): 243-248.
[17] 王彦, 许恒勇, 朱庆. 哺乳动物 DGAT 基因及其生物学功能研究进展. 遗传, 2007, 29(10): 1167-1172.
[18] Qi M, Lei T, Zhou L, Chen XD, Long H, Long QQ, Zhang RR, Yang ZQ, Gan L. Cloning, characterization, chromosomal mapping and tissue transcription analysis of porcine CREB2 and CREB3 genes. Folia Biol, 2009, 55(4): 137-144.
[19] Szczerbal I, Lin L, Stachowiak M, Chmurzynska A, Mackowski M, Winter A, Flisikowski K, Fries R, Switon-ski M. Cytogenetic mapping of DGAT1, PPARA, ADIPOR1 and CREB genes in the pig. J Appl Genet, 2007, 48(1): 73-76.
[20] 刘祖洞. 遗传学 (第二版). 北京: 高等教育出版社, 1990: 6-17.
[21] 张凯, 顾以韧, 刘艾晶, 蒋岸岸, 帅素容, 李明洲, 李学伟. 长白猪和梅山猪脂肪中脂肪沉积相关基因的表达差异. 畜牧兽医学报, 2010, 41(2): 129-134.

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猪ATF4基因多态性与生产性状的关联及基因表达分析

Expression analysis of porcine ATF4 gene and its association with production traits

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