遗传 ›› 2013, Vol. 35 ›› Issue (2): 185-191.doi: 10.3724/SP.J.1005.2013.00185

• 研究报告 • 上一篇    下一篇

山羊GOLA-DQA2基因多态性与血液免疫性状的相关分析

邢凤1, 秦孜娟2, 王桂芝2, 纪志宾2, 王建民2   

  1. 1. 塔里木大学动物科学学院,阿拉尔 843300 2. 山东农业大学动物科技学院,泰安 271018
  • 收稿日期:2012-08-28 修回日期:2012-11-20 出版日期:2013-02-20 发布日期:2013-02-25
  • 通讯作者: 王建民 E-mail:wangjm@sdau.edu.cn
  • 基金资助:

    山东省农业良种工程项目(编号:2011186)和山东省现代农业产业技术体系(编号:SATS2012263)资助

Association of polymorphisms of exon 2 of GOLA-DQA2 gene with blood immune traits in goats

XING Feng1, QIN Zi-Juan2, WANG Gui-Zhi2, JI Zhi-Bin2, WANG Jian-Min2   

  1. 1. College of Animal Science, Tarim University, Alar 843300, China 2. College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018, China
  • Received:2012-08-28 Revised:2012-11-20 Online:2013-02-20 Published:2013-02-25

摘要: 文章采用PCR-RFLP技术对莱芜黑山羊、波尔山羊、鲁波山羊3个山羊种群的GOLA-DQA2基因外显子2进行遗传多态性研究, 并对其血液免疫指标的效应进行分析。结果表明, 3个山羊种群共检测到4种基因型, 由3个等位基因控制; GOLA-DQA2基因外显子2的第77、79、80和169位的碱基表现出多态性; 多数血液免疫指标品种效应是主要效应; 鲁波山羊中, AB基因型的红细胞计数、白细胞计数分别显著高于BB基因型、BC基因型(P<0.05); AB基因型的红细胞压积显著高于BB、BC 基因型(P<0.05); BC基因型的噬中性粒细胞比率显著高于BB基因型(P<0.05); 波尔山羊和莱芜黑山羊中, 基因型与血液免疫指标之间也有一定的相关性, 但没有达到显著水平。由上述结果初步推测, 在鲁波山羊中, AB、BC基因型是影响红细胞计数、白细胞总数、噬中性粒细胞比率等血液免疫指标的重要因素。研究结果揭示GOLA-DQA2基因与血液免疫指标之间有一定的相关性, 可为山羊的抗病育种提供一定的参考。

关键词: 山羊, MHC-DQA2, PCR-RFLP, 多态性, 免疫性状

Abstract: The polymorphisms of exon 2 of GOLA-DQA2 gene were analyzed by PCR-RFLP technique in Laiwu black goats, Lubo goats, and Boer goats; and the effects of genotypes of GOLA-DQA2 gene on blood immune traits were esti-mated. The results showed that four genotypes were detected in the three goats. Polymorphic sites were detected at base positions 77, 79, 80, and 169 in exon 2 of GOLA-DQA2 gene. The effects of breed were major effect. In Lubo goats, red blood count (RBC) with genotype AB was significantly higher than that with genotype BB (P<0.05), and white blood count (WBC) with genotype AB was significantly higher than that with genotype BC (P<0.05). Hematocrit (HCT) in Lubo goats with genotype AB was significantly higher than that with genotypes BB and BC (P<0.05). WBC-large cell ratio (W-LCR) in Lubo goats with genotype BC was significantly higher than that with genotype BB (P<0.05). In Laiwu black goats and Boer goats, there was certain degree of association between genotypes and blood immune traits, but it was not significant. Genotype AB and BC was an important factor affecting RBC, WBC, W-LCR, and so on. It was concluded from these results that GOLA-DQA2 was the gene affecting the blood immune traits, which provides a basis for disease resistance breeding of goats.

Key words: goats, MHC-DQA2, PCR-RFLP, polymorphism, immune trait