山羊GOLA-DQA2基因多态性与血液免疫性状的相关分析

doi:10.3724/SP.J.1005.2013.00185

遗传 ›› 2013, Vol. 35 ›› Issue (2): 185-191.doi: 10.3724/SP.J.1005.2013.00185

山羊GOLA-DQA2基因多态性与血液免疫性状的相关分析

邢凤¹, 秦孜娟², 王桂芝², 纪志宾², 王建民²

1. 塔里木大学动物科学学院,阿拉尔 843300 2. 山东农业大学动物科技学院,泰安 271018

收稿日期:2012-08-28 修回日期:2012-11-20 出版日期:2013-02-20 发布日期:2013-02-25
通讯作者: 王建民 E-mail:wangjm@sdau.edu.cn
基金资助:
山东省农业良种工程项目(编号：2011186)和山东省现代农业产业技术体系(编号：SATS2012263)资助

Association of polymorphisms of exon 2 of GOLA-DQA2 gene with blood immune traits in goats

XING Feng¹, QIN Zi-Juan², WANG Gui-Zhi², JI Zhi-Bin², WANG Jian-Min²

1. College of Animal Science, Tarim University, Alar 843300, China 2. College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018, China

Received:2012-08-28 Revised:2012-11-20 Online:2013-02-20 Published:2013-02-25

摘要/Abstract

摘要： 文章采用PCR-RFLP技术对莱芜黑山羊、波尔山羊、鲁波山羊3个山羊种群的GOLA-DQA2基因外显子2进行遗传多态性研究, 并对其血液免疫指标的效应进行分析。结果表明, 3个山羊种群共检测到4种基因型, 由3个等位基因控制; GOLA-DQA2基因外显子2的第77、79、80和169位的碱基表现出多态性; 多数血液免疫指标品种效应是主要效应; 鲁波山羊中, AB基因型的红细胞计数、白细胞计数分别显著高于BB基因型、BC基因型(P<0.05); AB基因型的红细胞压积显著高于BB、BC 基因型(P<0.05); BC基因型的噬中性粒细胞比率显著高于BB基因型(P<0.05); 波尔山羊和莱芜黑山羊中, 基因型与血液免疫指标之间也有一定的相关性, 但没有达到显著水平。由上述结果初步推测, 在鲁波山羊中, AB、BC基因型是影响红细胞计数、白细胞总数、噬中性粒细胞比率等血液免疫指标的重要因素。研究结果揭示GOLA-DQA2基因与血液免疫指标之间有一定的相关性, 可为山羊的抗病育种提供一定的参考。

关键词: 山羊, MHC-DQA2, PCR-RFLP, 多态性, 免疫性状

Abstract: The polymorphisms of exon 2 of GOLA-DQA2 gene were analyzed by PCR-RFLP technique in Laiwu black goats, Lubo goats, and Boer goats; and the effects of genotypes of GOLA-DQA2 gene on blood immune traits were esti-mated. The results showed that four genotypes were detected in the three goats. Polymorphic sites were detected at base positions 77, 79, 80, and 169 in exon 2 of GOLA-DQA2 gene. The effects of breed were major effect. In Lubo goats, red blood count (RBC) with genotype AB was significantly higher than that with genotype BB (P<0.05), and white blood count (WBC) with genotype AB was significantly higher than that with genotype BC (P<0.05). Hematocrit (HCT) in Lubo goats with genotype AB was significantly higher than that with genotypes BB and BC (P<0.05). WBC-large cell ratio (W-LCR) in Lubo goats with genotype BC was significantly higher than that with genotype BB (P<0.05). In Laiwu black goats and Boer goats, there was certain degree of association between genotypes and blood immune traits, but it was not significant. Genotype AB and BC was an important factor affecting RBC, WBC, W-LCR, and so on. It was concluded from these results that GOLA-DQA2 was the gene affecting the blood immune traits, which provides a basis for disease resistance breeding of goats.

Key words: goats, MHC-DQA2, PCR-RFLP, polymorphism, immune trait

邢凤，秦孜娟，王桂芝，纪志宾，王建民. 山羊GOLA-DQA2基因多态性与血液免疫性状的相关分析[J]. 遗传, 2013, 35(2): 185-191.

XING Feng QIN Zi-Juan WANG Gui-Zhi JI Zhi-Bin WANG Jian-Min. Association of polymorphisms of exon 2 of GOLA-DQA2 gene with blood immune traits in goats[J]. HEREDITAS, 2013, 35(2): 185-191.

参考文献

[1] Erlich H, Valdes AM, Noble J, Carlson JA, Varney M, Concannon P, Mychaleckyj JC, Todd JA, Bonella P, Fear AL, Lavant E, Louey A, Moonsamy P. HLA DR-DQ hap-lotypes and genotypes and type 1 diabetes risk: analysis of the type 1 diabetes genetics consortium families. Diabetes, 2008, 57(4): 1084-1092.
[2] 张夫千, 郑小敏, 唐大伟, 赵鹏, 史远刚. 荷斯坦牛BoLA-DRB3基因多态性及其与乳房炎抗性关系分析. 畜牧兽医学报, 2007, 38(2): 115-119.
[3] Aarestrup FM, Jensen NE, Ostergard H. Analysis of asso-ciations between major histocompatibility complex (BOLA) class I haplotypes and subclinical mastitis of dairy cows. J Dairy Sci, 1995, 78(8): 1684-1692.
[4] 高树新, 许尚忠, 李金泉, 高雪, 任红艳, 陈金宝, 马云. BoLA-DQA, DRB3 exon 2多态性及其与奶牛乳房炎的关联分析. 畜牧兽医学报, 2006, 37(4): 317-320.
[5] 申红,贾斌,余智勇, 陈明辉, 刘宏坤, 李海, 陈玉林, 彭林泽, 林迎春. 多浪羊和中国美利奴羊MHC-DRB1基因多态性与包虫病的遗传易感性. 中国人畜共患病学报, 2007, 23(10): 1004-1008.
[6] Sayers G, Good B, Hanrahan JP, Ryan M, Angles JM, Sweeney T. Major histocompatibility complex DRB1 gene: its role in nematode resistance in Suffolk and Texel sheep breeds. Parasitology, 2005, 131(3): 403-409.
[7] Woodall CJ, Maclaren LJ, Watt NJ. Differential levels of mRNAs for cytokines, the interleukin -2 receptor and class II DR/DQ genes in ovine interstitial pneumonia induced by maedi visna virus infection. Vet Pathol, 1997, 34(3): 204-211.
[8] Zhou H, Hickford JG. Rapid communication: three new allelic sequences at the ovine MHC class II DQA1 locus. J Anim Sci, 2001, 79(3): 779-780.
[9] Zhou H, Hickford JG. Allelic polymorphism in the ovine DQA1 gene. J Anim Sci, 2004, 82 (1): 8-16.
[10] Hickford JG, Zhou H, Slow S, Fang Q. Diversity of the ovine DQA2 gene. J Anim Sci, 2004, 82(6): 1553-1563.
[11] Takeshima S, Chen S, Miki M, Kado M, Aida Y. Distri-bution and origin of bovine major histocompatibility complex class II DQA1 genes in Japan. Tissue Anti-gens, 2008, 72(3): 195- 205.
[12] Amills M, Francino O, Sánchez A. Nested PCR allows the characterization of Taq I and Pst I RFLPs in the second exon of the Caprine MHC class II DRB gene. Vet Immunol Immunopathol, 1995, 48(3-4): 313-321.
[13] Amills M, Francino O, Sànchez A. A PCR-RFLP typing method for the Caprine MHC class II DRB gene. Vet Immunol Immunopathol, 1996, 55(1-3): 255-260.
[14] 孙东晓, 张沅, 李宁. 蒙古山羊和哈萨克山羊GOLA- DRB3基因的Hae III酶切多态性分析. 遗传, 2004, 26(1): 55-58.
[15] Li MH, Li K, Kantanen J, Feng Z, Fan B, Zhao SH. Alle-lic variations in exon 2 of caprine MHC class II DRB3 gene in Chinese indigenous goats. Small Ruminant Res, 2006, 66(1-3): 236- 243.
[16] 杨易, 徐金瑞, 王杰, 杨雪, 郑玉才. 四川4个山羊品种(群体)MHC-DRB3外显子2的多态性. 黑龙江畜牧兽医, 2006, (1): 12-15.
[17] Amills M, Sulas C, Sànchez A, Bertoni G, Zanoni R, Obexer-Ruff G. Nucleotide sequence and polymorphism of the caprine major histocompatibility complex class II DQA1 (Cahi-DQA1) gene. Mol Immunol, 2005, 42(3): 375-379.
[18] 邢凤. 山羊DQA1、DQA2、DQB1基因多态性及与线虫抗性、免疫性状的相关分析[学位论文]. 泰安:山东农业大学, 2008.
[19] 李俊营, 陈宏, 蓝贤勇, 闵令江, 雷初朝, 胡沈荣. 5个山羊品种GoLA-DQA1基因的多态性分析. 西北农林科技大学学报, 2008, 36(1): 33-37.
[20] 柴文琼. 绒山羊DQA1基因第2外显子遗传特征分析[学位论文]. 兰州: 甘肃农业大学, 2012.
[21] Zhou H, Hickford JG, Fang Q. Polymorphism of the DQA2 gene in goats. J Anim Sci, 2005, 83(5): 963-968.
[22] 刘云芳, 剡根强, 王新峰. 多浪羊MHC- DRB3 基因座的PCR-RFLP 多态性分析. 遗传, 2004, 26(1): 59- 62.
[23] Andersson L, Rask L. Characterization of the MHC class II region in cattle. The number of DQ genes varies between haplotypes. Immunogenetics, 1988, 27(2): 110-120.
[24] 邢凤, 李培培, 李珏, 李成渤, 王建民. 山羊GOLA-DQB1基因外显子2多态性与免疫性状的相关分析. 遗传, 2008, 30(7): 870-876.
[25] 萨姆布鲁克J, 拉塞尔著DW. 分子克隆实验指南(第三版) (黄培堂译). 北京: 科学出版社, 2008: 196- 201.
[26] Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet, 1980, 32(3): 314-331.
[27] Blat

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

山羊GOLA-DQA2基因多态性与血液免疫性状的相关分析

Association of polymorphisms of exon 2 of GOLA-DQA2 gene with blood immune traits in goats

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	宋绍征, 何正义, 成勇, 于宝利, 张婷, 李丹. TALENs介导MSTN基因突变山羊的制备及性能分析[J]. 遗传, 2022, 44(6): 531-542.
[2]	王雪倩, 张庆珍, 程鹏, 董婷婷, 李卫国, 周喆, 王升启. 中国汉族人群66个InDel基因座的遗传多态性[J]. 遗传, 2022, 44(4): 335-345.
[3]	李以格, 张丹丹. 后GWAS时代结直肠癌致病SNP功能机制的研究进展[J]. 遗传, 2021, 43(3): 203-214.
[4]	王浩宇, 胡渝涵, 曹悦岩, 朱强, 黄雨果, 李茜, 张霁. 基于全基因组数据的AI-SNPs筛选及大陆次级区域内群体遗传结构差异研究[J]. 遗传, 2021, 43(10): 938-948.
[5]	王玉琢, 张一鸣, 董晓莲, 王学才, 朱建福, 王娜, 江峰, 陈跃, 姜庆五, 付朝伟. 2型糖尿病易感基因SNP位点对生活方式干预降低血糖应答效果的修饰效应[J]. 遗传, 2020, 42(5): 483-492.
[6]	宋绍征, 于康英, 张婷, 陆睿, 潘生强, 周鸣鸣, 成勇. tPA/gGH双基因转染山羊乳腺上皮细胞表达分析[J]. 遗传, 2020, 42(4): 380-387.
[7]	王冰源, 牟玉莲, 李奎, 刘志国. 农业动物干细胞研究进展[J]. 遗传, 2020, 42(11): 1073-1080.
[8]	梁文权,侯豫,赵存友. 精神分裂症相关单核苷酸多态性调控microRNA功能研究进展[J]. 遗传, 2019, 41(8): 677-685.
[9]	张晴,平杰,张昊翔,康波,李元丰,周钢桥. MKL1基因多态性与高原环境适应性的遗传关联研究[J]. 遗传, 2019, 41(7): 634-643.
[10]	王凤红,张磊,李晓凯,范一星,乔贤,龚高,严晓春,张令天,王志英,王瑞军,刘志红,王志新,何利兵,张燕军,李金泉,赵艳红,苏蕊. 山羊基因组研究进展[J]. 遗传, 2019, 41(10): 928-938.
[11]	张婕妤,初亚男,邹秉杰,张晏洁,封利颖. 基于级联核酸侵入反应的real-time PCR法检测咽拭子样本中UGT1A16*基因多态性[J]. 遗传, 2018, 40(8): 668-675.
[12]	彭哲也,唐紫珺,谢民主. 机器学习方法在基因交互作用探测中的研究进展[J]. 遗传, 2018, 40(3): 218-226.
[13]	刘莉莉, 郭爱伟, 吴培福, 陈粉粉, 杨亚晋, 张勤. 敲降VPS28基因对中国荷斯坦奶牛乳脂合成的调控[J]. 遗传, 2018, 40(12): 1092-1100.
[14]	李俊涛,赵薇,李丹丹,冯静,巴贵,宋天增,张红平. miR-101a靶向EZH2促进山羊骨骼肌卫星细胞的分化[J]. 遗传, 2017, 39(9): 828-836.
[15]	杨熳,卢冰婕,段媛媛,陈晓峰,马建岗,郭燕. 骨质疏松症易感基因BDNF的遗传学关联分析及功能研究[J]. 遗传, 2017, 39(8): 726-736.