关键词: PCR-RFLP, 免疫性状, 相关分析, MHC-DQB1, 山羊

Abstract:

PCR-RFLP was used to analyze the polymorphisms of exon 2 of GOLA-DQB1 gene in Laiwu black goats, Lubo goats, and Boer goats. The effects of genotypes of exon 2 of GOLA-DQB1 gene on immune traits were estimated. Seven genotypes (AA, BB, CC, AB, AC, BC, and DD) were detected by analyzing restriction maps. Polymorphic sites were detected at base position 24, 151 of exon 2 of GOLA-DQB1 gene. The results showed that the effects of breed were major effect. W-SCR in Laiwu black goats with genotype BC was significantly higher than that with genotype AC and CC (P<0.05), W-LCR in Laiwu black goats with genotype BC was significantly lower than that with genotype CC (P<0.05); W-LCC in Laiwu black goats with genotype BC was lower than that with genotype AC, CC (P>0.05). W-LCC in Boer goats with genotype BC was lower than that with genotype AA, AB, and BB (P>0.05); W-LCC in Lubo goats with genotype BC and AC was significantly lower than that with genotype AA (P<0.05). It was concluded from the results that GOLA-DQB1 was the gene affecting the immune traits. These results may be applied to marker assisted selection in disease resistance breeding of goats.