遗传 ›› 2003, Vol. 25 ›› Issue (2): 146-150.

• 论文 • 上一篇    下一篇

猪防御素基因PBD-I在大肠杆菌中的重组和融合表达

罗刚;魏泓   

  1. 第三军医大学实验动物中心,重庆 400038
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-04-10 发布日期:2003-04-10

Recombination and Fusion Expression of Porcine Defensin Gene PBD-I in E.coli

LUO Gang;WEI Hong   

  1. Laboratory Animal Center,Third Military Medical University,Chongqing 400038,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-04-10 Published:2003-04-10

摘要: 用RT-PCR方法获得PBD-I基因,将该基因插入原核表达载体PinPointTMXa-3中,重组质粒转化大肠杆菌JM109,经IPTG诱导后以融合蛋白形式表达。SDS-PAGE电泳显示PBD-I基因在目标位置17kDa处获得了表达。PBD-I基因的成功表达为进一步研究其抗菌活性、抗菌机理及应用研究形成基础。

关键词: 融合, 基因重组, 猪防御素

Abstract: The porcine defensin gene PBD-I was amplified by RT-PCR,then the gene was inserted into expression vector PinPointTM Xa-3.Recombinant plasmid named as ppd-1 was transformed into E.Coli JM109,which could effectively produce fusion protein induced with IPTG.The positive clone of PBD-I gene expressed 17kDa fusion protein by SDS-PAGE electrophoresis.Expression of PBD-I gene didn't increase distinctly along with time.The expression of PBD-I gene lays a foundation in research on antimicrobial activities and its mechanism of the defensin.