遗传 ›› 2003, Vol. 25 ›› Issue (3): 261-266.
姜劲迈;潘凯枫;马峻岭;宁涛;路桂荣;游伟程;柯杨 JIANG Jin-Mai;PAN Kai-Feng;MA Jun-Ling;NING Tao;LU Gui-Rong;YOU Wei-Cheng;KE Yang
摘要: 为探讨宿主的遗传背景和幽门螺杆菌(Helicobacter pylori,H.pylori)相关胃癌的易感性之间的关系,本文采用PCR产物直接测序和PCR-RFLP的方法,检测142例山东临朐县胃癌高发人群个体(包括69例癌症患者和73例非癌个体)和93例北京正常对照个体SE基因多态性的分布特点。结果显示:sew/sew基因型在山东非癌个体和北京人群之间的分布差异具有统计学意义(P<0.01,OR=3.06,95% CI,1.28~7.30),sew/sew基因型在山东癌症病人和非癌个体之间分布频率无显著性差异,H.pylori感染状况与SE基因型的分布也无关联性。提示:sew/sew纯合突变在山东临朐人群中分布频率较高,可能为临朐人群的遗传标记之一。
Abstract:To study the relation between host genetic backgroud and the susceptibility to H.pylori associated gastric cancer,PCR-sequencing and PCR-RFLP were used to screen SECRETOR gene polymorphisms in 142 subjects including 69 cancer patients and 73 non-cancer individuals from high-risk area of gastric cancer in Shandong and 93 control individuals from Beijing.Results showed that the difference in sew/sew distribution between non-cancer individuals and Beijing population was significant(P<0.01,OR is 3.06,95% CI,1.28~7.30),but that between cancer patients and non-cancer individuals was not with significance.SE gene polymorphism was not relevant to H.pylori infection.We concluded that Shandong population from high-risk area of gastric cancer shared a high distribution of sew/sew genotype,which could be considered as one of the genetic markers.