遗传 ›› 2006, Vol. 28 ›› Issue (8): 977-983.

• 研究报告 • 上一篇    下一篇

森林生态系中球孢白僵菌遗传多样性的ISSR分析

李 旻1, 王四宝1, 2, 樊美珍1, 李增智1, 黄勇平2   

  1. 1. 安徽农业大学安徽省微生物防治重点实验室, 合肥 230036;
    2. 中国科学院上海生命科学院植物生理生态研究所, 上海 200032

  • 收稿日期:2005-12-08 修回日期:2006-02-06 出版日期:2006-08-10 发布日期:2006-08-10
  • 通讯作者: 李 旻

Genetic Diversity of Beauveria bassiana (Bals.) Vuill. in Forest Ecosystem Assessed by Inter-simple Sequence Repeat (ISSR) Markers

LI Min 1, WANG Si-Bao 1,2, FAN Mei-Zhen 1,LI Zeng-Zhi 1, HUANG Yong-Ping 2   

  1. 1. Anhui Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036,China;
    2 Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032,China

  • Received:2005-12-08 Revised:2006-02-06 Online:2006-08-10 Published:2006-08-10
  • Contact: LI Min

摘要:

应用ISSR分子标记对安徽大别山区的球孢白僵菌遗传多样性进行了研究。从33个引物中筛选出12个多态性高、稳定性好的ISSRs用于正式的扩增分析,在2个自然保护区、3个不同季节和3个不同海拔梯度采集的48个菌株中共扩增出84条带,其中73条为多态性条带,多态性为81%,平均每个引物扩增出7条(2~11)。群体的多态位点百分率(PPL)达81%,Nei’s基因多样性(H)为0.3187,Shannon信息指数(I)为 0.4782。居群间的基因分化系数较小(Gst)0.1028。以上结果表明:安徽大别山区球孢白僵菌有较高的遗传多样性, 居群间遗传变异较小,居群内表现出较高水平的遗传分化。

关键词: 分子标记, 虫生真菌, 遗传多态性, 遗传分化

Abstract:

In the present paper, the genetic diversity of 48 Beauveria bassiana strains from different altitudes and at different seasons in Dabie Mountains of western Anhui was estimated using inter-simple sequence repeat (ISSR) markers. Twelve among 33 ISSR primers were chosen for their reproducibility and high polymorphism. Seven (2~11) markers per primer were scored, and a total of 84 fragments were amplified, in which 73 (81%) were polymorphic. Genetic diversity analysis revealed a relatively high level of intraspecific genetic diversity of B. bassiana in Dabie Mountains of western Anhui: the percentage of polymorphic loci (PPL) was 81%, Nei′s genetic diversity (He) was 0.3187 and Shannon′s genetic diversity index (I) was 0.4782. The genetic differentiation, GST was 0.1028, indicating that a low degree of genetic differentiation occurred in the B. bassiana among populations.

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