关键词: 多态性, 2型糖尿病, 脂蛋白脂酶基因

Abstract:

We examined the association of a HindⅢ RFLP (restriction fragment length polymorphism) in the lipoprotein lipase (LPL) gene with type 2 diabetes (T2DM) in Chinese Han population in Hubei Province. Genotypes were determined by PCR-RFLP in 102 controls and 264 T2DM patients using sib-pair and unrelated case-control designs. The frequencies of the H+ allele and H+H+ genotype for patients were significantly higher than those for controls (H+: 76.9% vs 69.1%, P<0.05; H+H+: 59.8% vs 52%, P<0.05). When all subjects were grouped as designed, the H+ allele and H+H+ genotype for sib patients were significantly higher than those for sib controls (H+: 81.5% vs 67.8%, P<0.05; H+H+: 68.5% vs 50.7%, P<0.05), while there were no significant differences in controls and random patients (P>0.05). Logistic regression analysis suggested that risk factors for T2DM was fasting plasma glucose and LPL genotypes, with individuals with the H+H+ genotype doubling their risk for T2DM as compared to those with the H+H- and H-H- genotypes (95% CI: 1.036～3.840, P<0.05). These data suggest that the HindⅢ RFLP in the LPL gene is associated with T2DM risk in Chinese Han popula-tion in Hubei Province, and the H+ allele may serve as a genetic risk factor of T2DM.