遗传 ›› 2009, Vol. 31 ›› Issue (4): 365-373.doi: 10.3724/SP.J.1005.2009.00365
侯琳1, 2;钱敏平1, 3;朱云平2;邓明华1, 3
1. 北京大学数学科学学院, 北京 100871;
2. 军事医学科学院放射与辐射医学研究所, 北京蛋白质组研究中心, 蛋白质组学国家重点实验室, 北京 100850;
3. 北京大学理论生物学中心, 北京 100871
HOU Lin1, 2;QIAN Min-Ping1, 3;ZHU Yun-Ping2;DENG Ming-Hua1, 3
摘要：
By using genome in situ hybridization (GISH) on root somatic chromosomes of allotetraploid derived from the cross Gossypium arboreum × G. bickii with genomic DNA (gDNA) of G. bickii as a probe, two sets of chromosomes, consisting of 26 chromosomes each, were easily distinguished from each other by their distinctive hybridization signals. GISH analysis directly proved that the hybrid G.arboreum×G. bickii is an allotetraploid amphiploid. The karyotype formula of the species was 2n = 4x = 52 = 46m (4sat) + 6sm (4sat). We identified four pairs of satellites with two pairs in each sub-genome. FISH analysis using 45S rDNA as a probe showed that the cross G. arboreum×G. bickii contained 14 NORs. At least five pairs of chromosomes in the G sub-genome showed double hybridization (red and blue) in their long arms, which indicates that chromatin introgression from the A sub-genome had occurred.
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