遗传 ›› 2009, Vol. 31 ›› Issue (4): 359-364.doi: 10.3724/SP.J.1005.2009.00359

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DArT技术的原理及其在植物遗传研究中的应用

洪义欢1;肖宁2;张超1;苏琰1;陈建民1   

  1. 1. 扬州大学生物科学与技术学院, 扬州 225009;
    2. 江苏里下河地区农业科学研究所, 扬州 225000
  • 收稿日期:2008-10-04 修回日期:2008-11-07 出版日期:2009-04-10 发布日期:2009-04-10
  • 通讯作者: 陈建民

Principle of diversity arrays technology (DArT) and its applications in genetic research of plants

HONG Yi-Huan1;XIAO Ning2;ZHANG Chao1;SU Yan1;CHEN Jian-Min1   

  1. 1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China;
    2. Agricultural Scicece Institute of Jiangsu Lixiahe District, Yangzhou 225000, China
  • Received:2008-10-04 Revised:2008-11-07 Online:2009-04-10 Published:2009-04-10
  • Contact: CHEN Jian-Min

摘要: DArT是一种依赖于基因芯片杂交基础之上的分子标记技术, 由于其具有省时、高通量、低花费、无需预知研究对象的DNA序列等优点而被应用到植物遗传研究中。文章介绍了DArT技术的原理、特点、具体操作方法及其目前在植物遗传研究中的应用。

关键词: 杂交, DArT技术, 分子标记

Abstract: Diversity arrays technology (DArT) is a microarray hybridization-based molecular marker technique, and a novel method to discover genetic polymorphic markers. DArT has recently been applied in genetic research of plants be-cause of its characteristics of sequence-independent, high–throughput, fast, low-cost. We briefly introduce the principle, characteristics, protocol of DArT and its application in genetic research of plants.