褪黑激素对绒山羊皮肤中毛囊周期相关miRNAs表达模式的影响

doi:10.3724/SP.J.1005.2014.1235

遗传 ›› 2014, Vol. 36 ›› Issue (12): 1235-1242.doi: 10.3724/SP.J.1005.2014.1235

褪黑激素对绒山羊皮肤中毛囊周期相关miRNAs表达模式的影响

付绍印^{1, 2, 3, 4}, 赵宏丽¹, 郑竹清^{1, 2}, 李金泉^{1, 2}, 张文广^{1, 2, 3}

1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
2. 动物遗传育种与繁殖内蒙古自治区重点实验室, 呼和浩特 010018;
3. 内蒙古ATCG生物信息研究所, 呼和浩特 010020;
4. 内蒙古自治区农牧业科学院, 呼和浩特 010031

收稿日期:2014-05-04 出版日期:2014-12-20 发布日期:2014-12-20
通讯作者: 张文广, 教授, 博士生导师, 研究方向：数量基因组学暨性状遗传机理。E-mail: atcgnmbi@aliyun.com E-mail:fushao1234@126.com
作者简介:付绍印, 博士研究生, 专业方向：动物遗传育种与繁殖。
基金资助:
国家科技支撑项目(编号：2011BAD28B05)和国家自然科学基金项目(编号：31260538)资助

Melatonin regulating the expression of miRNAs involved in hair folli-cle cycle of cashmere goats skin

Shaoyin Fu^{1, 2, 3, 4}, Hongli Zhao¹, Zhuqing Zheng^{1, 2}, Jinquan Li^{1, 2}, Wenguang Zhang^{1, 2, 3}

1.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Autonomous Region, Hohhot 010018, China;
3. Institute of ATCG, Nei Mongolia Bio-Information, Hohhot 010020, China;
4. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China

Received:2014-05-04 Online:2014-12-20 Published:2014-12-20

摘要/Abstract

摘要： 褪黑激素(Melatonin, MT)和miRNAs在毛囊的生长发育过程中发挥重要的作用, 但MT对绒山羊皮肤毛囊miRNAs表达模式的影响尚未见报道。为探索MT从miRNAs层次影响山羊绒生长的机制, 文章在内蒙古绒山羊中实施了褪黑激素埋植试验：5只青年母羊作为实验组埋植褪黑激素, 另外5只青年母羊作为对照。利用荧光定量PCR检测褪黑激素埋植前后毛囊周期相关miRNAs的表达变化。结果表明, 埋植MT明显改变了6个绒毛相关miRNAs的表达规律：在一个绒毛周期内, 除let-7a外, miR-203、miR-205、miR-96、miR-183和miR-199a的表达量均发生3次跃迁;埋植MT改变了miRNAs之间的共表达模式。对照组各miRNA之间相关系数范围为0.87~0.99(P<0.01)。与对照组相比, 埋植组中let-7a与miR-96、miR-199a、miR-205, miR-203与miR-96、miR-199a, miR-96与miR-183, miR-183与miR-199a之间的相关系数被明显消弱;MT通过下调6月份埋植组各miRNA的表达量提早诱发二次生绒。

关键词: 褪黑激素, miRNAs, 绒山羊, 绒毛周期

Abstract: Melatonin and microRNAs (miRNAs) play important roles in regulating hair follicle development. However, the effect of melatonin on the expression pattern of miRNAs in skin and follicle of cashmere goats remain largely undefined. To explore the mechanism of melatonin affecting cashmere growth mediated by miRNAs, the effect of melatonin implants administered in Nei Mongol cashmere goats was assessed. In the experiment, five yearling does were implanted with melatonin, with the remaining other five females as control group. The expression of six candidate miRNAs was quantified by reverse transcription-real time polymerase chain reaction (RT-qPCR). The results indicated that melatonin significantly altered the expression pattern of miRNAs. Except for let-7a, the expression levels of miR-203, miR-205, miR-96, miR-183 and miR-199a occur three transitions during a cashmere cycle; melatonin changed the co-expression pattern of miRNAs. The correlation coefficient between miRNAs is 0.87-0.99 in control group(P<0.01). Compared with the control group, the correlation coefficient between some miRNAs (let-7a and miR-96, miR-199a, miR-205;miR-203 and miR-96, miR-199a; miR-96 and miR-183; miR-183 and miR-199a) was significantly weakened in melatonin group. Melatonin might induce second growth of cashmere mediated by down-regulating the expression level of some miRNAs in June in melatonin implanted group.

Key words: melatonin, miRNAs, cashmere goats, cashmere cycle

付绍印, 赵宏丽, 郑竹清, 李金泉, 张文广. 褪黑激素对绒山羊皮肤中毛囊周期相关miRNAs表达模式的影响[J]. 遗传, 2014, 36(12): 1235-1242.

Shaoyin Fu, Hongli Zhao, Zhuqing Zheng, Jinquan Li, Wenguang Zhang. Melatonin regulating the expression of miRNAs involved in hair folli-cle cycle of cashmere goats skin[J]. HEREDITAS(Beijing), 2014, 36(12): 1235-1242.

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褪黑激素对绒山羊皮肤中毛囊周期相关miRNAs表达模式的影响

Melatonin regulating the expression of miRNAs involved in hair folli-cle cycle of cashmere goats skin

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