遗传 ›› 2006, Vol. 28 ›› Issue (10): 1254-1259.

• 研究论文 • 上一篇    下一篇

黑腹果蝇中一个Cy染色体上的缺失

李红伟1;连林生2;赵春江1;白丽华1;邓学梅1;吴常信1   

  1. 1. 中国农业大学动物科技学院, 北京 100094; 2. 云南农业大学动物科技学院, 昆明 650201
  • 出版日期:2006-10-01 发布日期:2006-10-01

Identification of a Common Deletion in Different Curly Mutants in Drosophila Melanogaster

LI Hong-Wei1; LIAN Lin-Sheng2; ZHAO Chun-Jiang1; BAI Li-Hua1;DENG Xue-Mei1; WU Chang-Xin1   

  1. 1. College of Animal Science and Technology, China Agricultural University, Beijing 100094, China; 2. College of Animal Science and Technology, Yunnan Agricultural University,Kunming 650201, China
  • Online:2006-10-01 Published:2006-10-01

摘要: 卷翅(Curly简称Cy)是易以识别的果蝇翅膀的显性突变, 是黑腹果蝇2号染色体上最常用的显性翅膀标记, 但对Cy的分子特征却不清楚。综合细胞遗传学与基因组学的信息,应用分子生物学技术,首次在Cy染色体上发现一个102 bp的缺失。该缺失存在于不同的卷翅品系中, 说明不同Cy染色体上存在某些共同的分子特征。同时以该缺失作为Cy染色体的DNA标记, 通过基因型多态分析, 初步证实Cy纯合型可导致果蝇胚胎期死亡。这些结果为进一步研究卷翅的分子遗传机理奠定了基础。

关键词: 黑腹果蝇, 卷翅, 缺失, 卷翅突变体

Abstract: Curly is a easily distinguishable dominant mutant wing character. The Cy mutation is the most commonly used dominant marker for the second chromosome balancers in Drosophila melanogaster, but little is known about the Cy gene. Based on known genomic and cytogenetic information, a 102 bp deletion which is located between the Genes synaptotagmin (syt) and Activin Like Protein at 23B(Alp23B) on the Drosophila melanogaster genome (release 4) had been found to be commonly contained on Cy chromosome in three different curly strains. Meanwhile, when using the deletion as a DNA marker, the result suggested that Cy homozygote be lethal in embryo period. These results will provide some helpful information to investigate molecular mechanism of curly wings in the further study.

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