遗传 ›› 2010, Vol. 32 ›› Issue (10): 1051-1056.doi: 10.3724/SP.J.1005.2010.01051

• 研究报告 • 上一篇    下一篇

黑腹果蝇3号染色体裂翅平衡致死位点的发现及2号、3号染色体裂卷翅双平衡染色体的建立

阎辉, 邓学梅, 吴常信   

  1. 中国农业大学动物科技学院, 北京 100193
  • 收稿日期:2010-01-21 修回日期:2010-04-06 出版日期:2010-10-20 发布日期:2010-10-20
  • 通讯作者: 吴常信 E-mail:chxwu@cau.edu.cn
  • 基金资助:

    “动物科学专业遗传学系列课程教学团队”设项目, “动物遗传学”国家级精品课程建设项目和中国农业大学URP计划项目资助

A new balancer of chapped wing on chromosome 3 and a gathering line with double balancers of chapped wing (L) and Cy in Drosophila melanogaster

YAN Hui, DENG Xue-Mei, WU Chang-Xin   

  1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
  • Received:2010-01-21 Revised:2010-04-06 Online:2010-10-20 Published:2010-10-20
  • Contact: WU Chang-Xin E-mail:chxwu@cau.edu.cn

摘要: 果蝇的平衡染色体在遗传研究中被广泛应用。文章通过分析黑腹果蝇裂翅新突变体与野生型、982紫眼及黑檀体杂交后代裂翅性状情况, 首次将裂翅基因定位于3号染色体上, 并阐明了裂翅平衡致死、杂合子纯繁的遗传机制, 获得了以裂翅为显性标记的3号平衡染色体品系。探索了双平衡染色体显性标记基因聚合的杂交模式, 成功建立了以裂翅和卷翅为标记的2号、3号双平衡染色体。裂翅的发现为3号染色体平衡子提供了更加方便识别的显性翅型标记, 同时裂卷翅双平衡体的建立丰富了果蝇常用工具平衡子, 可以广泛用于基因定位及突变筛选过程。

关键词: 裂翅, 平衡致死, 裂卷翅新品系, 果蝇

Abstract: The balancers of Drosophila melanogaster are widely used in genetic research. By analyzing the phenotype of offspring from hybridization of chapped wing (L) mating with OR, 982p and e, respectively, we mapped the chapped wing mutation on chromosome 3 for the first time and demonstrated the chapped wing mutation as a new balancer of D. melanogaster with dominant wing nicking phenotype. Finally, we bred a novel gathering line with double balancers of L and Cy in D. melanogaster. The mutant L provided a legible dominant marker for the balancer of chromosome 3, and the cultivation of double balancers chapped-curly wing enriches the balancer stock, which is often used in mapping and screening.

Key words: Drosophila melanogaster, chapped-wing, recessive lethalchapped-curly wing