小麦-黑麦附加系的创制及5R抗白粉病新基因的发现

doi:10.3724/SP.J.1005.2011.01258

遗传 ›› 2011, Vol. 33 ›› Issue (11): 1258-1262.doi: 10.3724/SP.J.1005.2011.01258

小麦-黑麦附加系的创制及5R抗白粉病新基因的发现

符书兰, 唐宗祥, 任正隆

四川农业大学植物遗传育种省级重点实验室, 温江 611130

收稿日期:2011-06-10 修回日期:2011-07-21 出版日期:2011-11-20 发布日期:2011-11-25
通讯作者: 符书兰 E-mail:fushulan@sicau.edu.cn
基金资助:
国家自然科学基金项目(编号：31000713), 国家高技术研究发展计划项目(863计划)(编号：2011AA100101)资助

Establishment of wheat-rye addition lines and De Novo powdery mildew resistance gene from chromosome 5R

FU Shu-Lan, TANG Zong-Xiang, REN Zheng-Long

State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang 611130, China

Received:2011-06-10 Revised:2011-07-21 Online:2011-11-20 Published:2011-11-25
Contact: FU Shu-Lan E-mail:fushulan@sicau.edu.cn

摘要/Abstract

摘要： 以重复序列pAS1和pSc119.2为探针, 对八倍体小黑麦×普通小麦的F₅代植株进行了FISH分析, 同时对这些材料进行了田间抗病性鉴定。从中鉴定出了1R、2R、3R、4R、5R、6R、7R单体附加系和1R、2R二体附加系, 1R和4R附加系出现频率相对较高。5R和6R单体附加系对白粉病免疫, 推测5R染色体上带有新的白粉病抗性基因。此外, 还检测到不少植株染色体组发生了变异, 且小麦4B染色体优先缺失。

关键词: 小麦, 黑麦, 附加系, 白粉病, 染色体缺失

Abstract: The F₅ plants derived from octoploid triticale × common wheat were investigated by FISH methods using repetitive DNA sequences pAS1 and pSc119.2 as probes. The disease resistance of these plants was also screened and evaluated in the field. The 1R, 2R, 3R, 4R, 5R, 6R and 7R monosomic addition lines and 1R and 2R disomic addition lines were found. The occurrence frequencies of chromosomes 1R and 4R addition lines were higher than that of chromosomes 2R, 3R, 5R, 6R, and 7R addition lines in the high generation screened. The 5R and 6R monosomic addition lines were im-mune to powdery mildew. The chromosome 5R in this study might carry new powdery mildew resistance gene(s). In addi-tion, the preferential elimination of chromosome 4B was observed in several plants.

Key words: wheat, rye, addition line, powdery mildew, chromosome elimination

符书兰，唐宗祥，任正隆. 小麦-黑麦附加系的创制及5R抗白粉病新基因的发现[J]. 遗传, 2011, 33(11): 1258-1262.

FU Shu-Lan, TANG Zong-Xiang, REN Zheng-Long. Establishment of wheat-rye addition lines and De Novo powdery mildew resistance gene from chromosome 5R[J]. HEREDITAS, 2011, 33(11): 1258-1262.

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小麦-黑麦附加系的创制及5R抗白粉病新基因的发现

Establishment of wheat-rye addition lines and De Novo powdery mildew resistance gene from chromosome 5R

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