遗传 ›› 2006, Vol. 28 ›› Issue (5): 623-630.

• 专论与综述 • 上一篇    下一篇

肽核酸—基因调控的利器

费一楠;张飞雄   

  1. 首都师范大学生命科学学院,北京 100037

  • 收稿日期:2005-08-17 修回日期:2005-12-07 出版日期:2006-05-10 发布日期:2006-05-10
  • 通讯作者: 张飞雄

Peptide Nucleic Acids(PNA) –The Explorer of Gene Mystery

FEI Yi-Nan, ZHANG Fei-Xiong

  

  1. College of Life Science , Capital Normal University, Beijing 100037, China

  • Received:2005-08-17 Revised:2005-12-07 Online:2006-05-10 Published:2006-05-10
  • Contact: ZHANG Fei-Xiong

摘要:

肽核酸(PNA)是具有类多肽骨架的DNA类似物,PNA的主链骨架是由N(2-氨基乙基)-甘氨酸与核酸碱基通过亚甲基羰基连接而成的。PNA可以特异性地与DNA或RNA杂交,形成稳定的复合体。PNA由于其自身的特点可以对DNA复制、基因转录、翻译等进行有针对的调控,同时作为杂交探针大大提高了遗传学检测和医疗诊断的效率和灵敏度。肽核酸(PNA)特异性地识别和结合互补核酸序列被引进用于医学和生物学的研究,展示了其独特的生化属性,成为了基因奥秘的探索者。 






关键词: 反义,  , PNA,  , 基因调控

Abstract: Peptide Nucleic Acids(PNA) are DNA analogues in which the naturally occurring nucleobases are attached via methylene carbonyl linkages to an achiral pseudopeptide backbone of N-(2-aminoethyl) glycine units. PNA can bind to both DNA and RNA targets in a sequence-specific manner. PNA provides a powerful tool to study the machanism of gene replication, transcription and an innovative strategy to regulate target gene expression. Advances in molecular biotechnology have greatly improved the sensitivity and the efficiency of methods utilized for genetic investigations and diagnosis. Peptide Nucleic Acids(PNA) which recognizes and binds to complementary nucleic acid sequence presents unique physicochemical properties and has been incorporated into an expanding set of biological investigations.

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