遗传 ›› 2007, Vol. 29 ›› Issue (8): 945-945―950.

• 研究报告 • 上一篇    下一篇

南阳牛DGAT2基因PCR-RFLP多态性及其与生长性状相关性研究

张争锋1,4, 陈宏1,2, 李秋玲1,5, 雷初朝1, 张春雷1, 王新庄3 , 王居强3, 王轶敏3   

  1. 1. 西北农林科技大学动物科技学院, 陕西省农业分子生物学重点实验室, 杨凌 712100;
    2. 徐州师范大学细胞与分子生物学研究所, 徐州 221116;
    3. 河南省肉牛工程技术研究中心, 郑州 450003;
    4. 山东省计算中心, 济南 250014;
    5. 山东省农业科学院奶牛研究中心, 济南 250100

  • 收稿日期:2007-01-09 修回日期:2007-03-05 出版日期:2007-08-10 发布日期:2007-08-10
  • 通讯作者: 张争锋

Polymorphisms of DGAT2 gene and its associations with several growth traits in Nanyang cattle

ZHANG Zheng-Feng1,4, CHEN Hong1,2, LI Qiu-Ling1,5, LEI Chu-Zhao1,
ZHANG Chun-Lei1, WANG Xin-Zhuang3, WANG Ju-Qiang3, W   

  1. 1. College of Animal Science and Technolog, Shaanxi Key Laboratory of Molecular Biology for Agricultue, Northwest A&F University, Yangling 712100, China;
    2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China;
    3. Research Center of Cattle Engineering Technology in Henan, Zhengzhou 450003, China;
    4. Shandong Computer Science Center, Ji’nan 250014 China;
    5. Dairy Cattle Rearch Center, Shandong Academy of Agricultural Science, Ji’nan 250100, China
  • Received:2007-01-09 Revised:2007-03-05 Online:2007-08-10 Published:2007-08-10
  • Contact: ZHANG Zheng-Feng

摘要:

以131头纯种南阳牛为研究材料, 利用PCR-RFLPs对二酰甘油酰基转移酶2(DGAT2)基因的第6内含子和第7内含子的多态性及其与生长发育的相关性进行了分析。结果表明: 南阳牛在该位点分别检测到两种等位基因A/B和N/M, 频率分别为0.875/0.125和0.971/0.029。A和N等位基因是群体中的优势等位基因。该基因内含子6对南阳牛6月龄的体高、2岁体重、6月龄到两岁的胸围和体斜长都有显著的影响, 内含子6的AA基因型的6月龄的体高比杂合型高3.8 %, 两岁体重高3.9%, 6月龄到两岁的胸围分别高3.8%、3.4%、3.7%、4.3%; 6月龄到两岁的体斜长分别高3.8%、3.6%、3.8%、3.1%。内含子7对18月龄和两岁的坐骨端宽有极显著的影响(P<0.01); 对两岁的胸围有显著的影响(P<0.05)。

关键词: DGAT2, 南阳牛, PCR-RFLP, 生长性状

Abstract:

The associations between SNPs in intron 6 and intron 7 of the bovine diacylglycerolacyltransferase (DGAT2) gene and growth traits in purebred Nanyang cattle (n=131) were reported in this study. Alleles detected in intron 6 were allele A and B with frequencies of 0.875 and 0.125, respectively; alleles detected in intron 7 were allele N and M with frequencies of 0.968 and 0.032, respectively. The animals with genotype AA in intron 6 showed 3.8% increases in body height (P < 0.01), 3.9% increases in body weight (P < 0.05), 3.8% (P < 0.05), 3.6% (P < 0.05), 3.8% (P < 0.01) and 3.1%( P < 0.01) increase in body length at age six-month to two-year compared with genotype AB, respectively. They also showed 3.8 % (P < 0.05), 3.4 % ( P < 0.01), 3.7% (P < 0.01) and 4.3% (P < 0.01) increases in heart girth at age six-month to two-year respectively. Animals with the NM genotype show significantly higher average heart girth at age two years (P < 0.05), and Hucklebone width (P < 0.01) at age eighteen-month (P < 0.01) and two-year (P < 0.01) compared with animals with the genotype NN, respectively.