遗传 ›› 2008, Vol. 30 ›› Issue (9): 1153-1156.doi: 10.3724/SP.J.1005.2008.01153

• 研究报告 • 上一篇    下一篇

贵州汉族人群HLA-DM基因多态性分析

蒋红梅; 王永霞; 王勇   

  1. 贵阳医学院临床微生物学和免疫学教研室, 贵阳550004

  • 收稿日期:2008-01-06 修回日期:2008-02-21 出版日期:2008-09-10 发布日期:2008-09-10
  • 通讯作者: 蒋红梅

HLA-DM polymorphisms in Guizhou Han individuals

JIANG Hong-Mei; WANG Yong-Xia; WANG Yong   

  1. Department of Clinical Microbiology and Immunology, Guiyang Medical College, Guiyang 550004, China
  • Received:2008-01-06 Revised:2008-02-21 Online:2008-09-10 Published:2008-09-10
  • Contact: JIANG Hong-Mei

摘要:

为了探讨贵州汉族人群HLA-DM基因多态性的分布情况, 采用PCR-RFLP法对125 例贵州汉族人进行HLA-DM基因分型。结果显示, 贵州汉族人群DMA*0101~0103等位基因频率依次是0.720、0.244、0.036, DMB*0101~0104等位基因频率分布依次是0.620、0.156、0.188和0.036; 贵州汉族人群中DMA的基因型以DMA*0101/0101和0101/0102为主, 而DMB的基因型以DMB*0101/0101、0101/0102和0101/0103为主。结果表明, HLA-DM基因多态性具有地区性、民族性的遗传特征。

关键词: HLA-DM, 基因多态性, 汉族人群

Abstract:

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to determine genetic polymorphisms at HLA-DMA/DMB loci in 125 healthy unrelated Han individuals from Guizhou Province. We found that the frequencies of DMA*0101, DMA*0102, and DMA*0103 alleles were 0.720, 0.242, and 0.036, respectively. The fre-quencies of DMB*0101, DMB*0102, DMB*0103, and DMB*0104 alleles were 0.620, 0.156, 0.188, and 0.036, respec-tively. Common genotypes for DMA were DMA*0101/0101 and 0101/0102. Common genotypes for DMB were DMB*0101/0101, 0101/0102, and 0101/0103. These results showed that the genetic polymorphism of HLA-DM gene in Han individuals from Guizhou Province is distinct from other regions.