遗传 ›› 2009, Vol. 31 ›› Issue (12): 1221-1225.doi: 10.3724/SP.J.1005.2009.01

• 研究报告 • 上一篇    下一篇

POMC基因多态性及其与南阳牛生长性状的相关分析

张春雷1, 王艳红1, 陈宏1, 雷初朝2, 房兴堂1, 王居强3, 马桂变3, 牛晖3, 肖杰3   

  1. 1. 徐州师范大学细胞与分子生物学研究所, 徐州 221116; 2. 西北农林科技大学动物科技学院, 陕西省农业分子生物学重点实验室, 杨凌 712100; 3. 河南省肉牛工程技术研究中心, 郑州450003; 4. 河南省平顶山市畜牧兽医工作站, 平顶山 467001
  • 收稿日期:2009-08-16 修回日期:2009-09-30 出版日期:2009-12-10 发布日期:2009-12-10
  • 通讯作者: 陈宏 E-mail:chenhong1212@263.net
  • 基金资助:

    国家高技术研究发展计划项目(编号:2006AA10Z197, 2008AA101010), 国家自然科学基金项目(编号:30771544, 30972080), 国家科技支撑计划(2006BAD01A10-5), 转基因生物新品种培育科技重大专项(编号:2009ZX08009-157B, 2008ZX08007-002), 江苏省高校自然科学基金(编号:09KJD180002),国家肉牛产业体系项目和陕西省“13115”科技创新工程项目重点专项(2008ZDKG-11)资助。

The polymorphism of bovine POMC gene and its association with the growth traits of Nanyang cattle

ZHANG Chun-Lei1, WANG Yan-Hong1, CHEN Hong1, LEI Chu-Zhao2, FANG Xing-Tang1, WANG Ju-Qiang3, MA Gui-Bian4, NIU Hui3, XIAO Jie3   

  1. 1. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China; 2. College of Animal Science and Technology, Northwest A& F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, 712100, China; 3. Research Center of Beef Engineering and Technology in Henan, Zhengzhou, 450003, China; 4. Pingdingshan Animal Husbandry Station of Henan Province, Pingdingshan, 467001, China
  • Received:2009-08-16 Revised:2009-09-30 Online:2009-12-10 Published:2009-12-10
  • Contact: CHEN Hong E-mail:chenhong1212@263.net

摘要:

为研究阿片黑皮质素前体(POMC)在动物采食和能量平衡调控中发挥重要作用, 文章采用PCR-SSCP结合DNA测序方法, 对秦川牛、南阳牛、郏县红牛、晋南牛、鲁西牛、安格斯牛和荷斯坦奶牛共计480头个体POMC基因的多态性进行研究, 并分析了多态位点与南阳牛生长性状的相关性。结果表明, 牛POMC基因3个位点中, 在3′侧翼区P3位点新发现3个连锁存在的SNP(811845 C>T、811821 T>C和811797 A>G, 与NW_928357对照)。POMC基因3′侧翼区多态位点与南阳牛6月龄体重和0~6月龄平均日增重显著相关, BB型个体显著大于AA型(P<0.05)。

关键词: 牛, POMC基因, 单核苷酸多态性, 生长性状, 体重 牛, POMC基因, 单核苷酸多态性, 生长性状, 体重

Abstract:

Pro-opiomelanocortin (POMC) plays an important role on animal ingestive behavior and energy homeostasis. Genetic variation of POMC was detected by PCR-SSCP and DNA sequencing techniques in 480 individuals from seven cattle breeds (i.e., Qinchuan, Nanyang, Jiaxian Red cattle, Jinnan, Luxi, Angus, and Chinese Holstein), and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth traits in Nanyang cattle. Three SNPs (811845 C>T, 811821 T>C and 811797 A>G, ref. NW_928357) in linkage were detected in P3 locus, which were located at the 3’ flanking region of bovine POMC gene. The body weight at 6-month-old and the average daily gain of 0~6-month-old were higher in Nanyang cattle with the genotype BB than with the genotype AA (P<0.05).

Key words: cattle, POMC gene, SNP, growth trait, body weight