遗传 ›› 2012, Vol. 34 ›› Issue (2): 145-156.doi: 10.3724/SP.J.1005.2012.00145
施季森, 王占军, 陈金慧
收稿日期:2011-09-07
修回日期:2011-11-02
出版日期:2012-02-20
发布日期:2012-02-25
通讯作者:
施季森
E-mail:jshi@njfu.edu.cn
基金资助:国家自然科学基金重点项目(编号：30930077), 国家自然科学基金青年项目(编号：30901156), 国家林业局948引进项目(编号：2009-4-24), 江苏高校自然科学基金项目(编号：09KJA220001)和江苏省高校优势学科建设工程项目(PAPD)资助
SHI Ji-Sen, WANG Zhan-Jun, CHEN Jin-Hui
Received:2011-09-07
Revised:2011-11-02
Online:2012-02-20
Published:2012-02-25
Contact:
Shi Ji-sen
E-mail:jshi@njfu.edu.cn
摘要： 近年来, 植物全基因组测序的结果正如雨后春笋般涌现, 木本植物全基因组测序也在紧锣密鼓地展开。但由于木本植物通常基因组较大, 基因组结构较为复杂, 在测序、测序后的组装、注释、功能分析等均存在较大的困难。在基因组测序分析的经费预算方面也存在着较大的压力。因此, 有必要对这方面的研究进展及其存在问题进行分析比较, 以提高林木全基因组研究方面的效率。文章在比较分析已经发展起来的3代基因测序技术(Sanger测序法、合成测序法和单分子测序法)的基础上, 选择4种已经公布的木本植物(杨树、葡萄、番木瓜、苹果), 从全基因组测序的研究背景、测序结果及应用的研究进展和存在问题等方面进行了述评, 对未来要开展的木本植物全基因组测序前的准备工作(材料选择、遗传图谱和连锁图谱的构建、测序技术的选择), 全基因组测序结果的生物信息学分析和应用进行了讨论。
施季森，王占军，陈金慧. 木本植物全基因组测序研究进展[J]. 遗传, 2012, 34(2): 145-156.
SHI Ji-Sen, WANG Zhan-Jun, CHEN Jin-Hui. Progress on whole genome sequencing in woody plants[J]. HEREDITAS, 2012, 34(2): 145-156.
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