遗传 ›› 1997, Vol. 19 ›› Issue (6): 7-11.
沈银柱1; 刘植义1; 何聪芬1; 黄占景1; 孟庆昌1; 柏峰1; 马闻师1; 赵松山2;陆莉2; 张焕英2 SHEN Yin-Zhu1;LIU Zhi-Yi1;HE Cong-Fen1;HUANG Zhan-Jing1MENG Qing-Chang;BAI Feng1;MA Wen-Shi1;ZHAO Song-Shan2;LU Li2;ZHANG Huan-Ying2
摘要： 不同基因型小麦花药愈伤组织对化学诱变剂的敏感性不同。选用对诱变剂敏感的类型,经EMS(甲基磺酸乙酯)诱变可以产生耐盐变异。实验获得的耐盐变异株, 离开盐胁迫3代后,经盐池鉴定后代中有52.9%的品系达到一级耐盐,表现了一定的遗传稳定性。耐盐品系的结实率也逐渐得到恢复,达到92.4%。利用其高代材料进行耐盐性的遗传分析结果表明,小麦的耐盐性不仅受核基因的控制,也受细胞质因子的影响。经酸性聚丙烯酰胺凝胶电泳分析,发现耐盐突变体特有的Rf值为0.19的醇溶蛋白能稳定遗传。
Abstract:The study discovered that the sensibilities of anther calli in different wheat genetypes to chemical mutagenic agent were different.The mutation-sensitive type might generate salt-tolerant variation after mutagenizing with EMS.The fourth generation of salt-tolerant plants which were planted for three generations in usual soil expressed tolerance stability after planting in the salt pond of a 0.45% salt concentration,52.9% of the lines in the offspring belonged to first grade of salt-tolerance,showing that the salt-tolerant variant had a certain stability.The fertility of salt-tolerant lines was gradually recovered and reached 92.4%.The high generation materials were used for genetic analysis of the salt-tolerance.The results indicated that the wheat salt-tolerant trait is not only controlled by nuclear gene,but also effected by cytoplasmic factor.In addition,A-PAGE(Acid-Polyacrylamide gel electrophoresis)discovered that the salt-resistant mutant had a unique gliadin band with a Rf value of 0.19,and that this unique band was stable inherited.
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