植物质体基因工程：新的优化策略及应用

doi:10.16288/j.yczz.15-018

遗传 ›› 2015, Vol. 37 ›› Issue (8): 777-792.doi: 10.16288/j.yczz.15-018

植物质体基因工程：新的优化策略及应用

周菲, 路史展, 高亮, 张娟娟, 林拥军

华中农业大学,作物遗传改良国家重点实验室,武汉 430070

收稿日期:2015-01-06 修回日期:2015-04-12 出版日期:2015-08-20 发布日期:2015-08-20
通讯作者: 林拥军,博士,教授,研究方向：转基因抗虫水稻培育。E-mail: yongjunlin@mail.hzau.edu.cn
作者简介:周菲,博士,副教授,研究方向：质体生物学及生物技术。E-mail: zhoufei@mail.hzau.edu.cn
基金资助:
国家自然科学基金青年项目(编号：31100945)和校自主科技创新基金项目(编号：2012ZYTS056)资助

Plastid genome engineering: novel optimization strategies and applications

Fei Zhou, Shizhan Lu, Liang Gao, Juanjuan Zhang, Yongjun Lin

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China

Received:2015-01-06 Revised:2015-04-12 Online:2015-08-20 Published:2015-08-20

摘要/Abstract

摘要： 植物质体转化技术通过同源重组实现定点整合,与细胞核基因工程相比,使外源基因表达更为精确、安全和高效。该技术在基础研究中为叶绿体功能基因组研究提供了有效手段,同时在应用方面为外源基因表达提供了理想的平台,已成为植物遗传育种的一种新策略。本文总结了近年来质体基因工程在转化体系的建立和优化上的新思路,着重阐述了利用质体转化技术在遗传育种中提高作物抗性、改良品质等应用领域的最新研究进展。克服质体转化技术在作物遗传育种中面临的难题,必将为作物育种的发展带来新的绿色革命。

关键词: 质体基因工程, 表达调控元件, 遗传育种

Abstract: The plastid genome engineering system allows site-specific modifications via two homologous recombination events. It is much safer, more precise and efficient compared with the nuclear transformation system. This technology can be applied to the basic research to expand plastid genome function analysis, and it also provides an excellent platform for not only high-level production of recombinant proteins but also plant breeding. In this review, we summarize the state of the art and progresses in this field. We focus on novel breeding strategies in transformation system improvement and new tools to enhance plastid transgene expression levels. In addition, we highlight selected applications in resistance engineering and quality improvement via metabolic engineering. We believe that by overcoming current technological limitations in the plastid transformation system can another green revolution for crop breeding beckon.

Key words: plastid genome engineering system, expression regulation element, genetic breeding

周菲, 路史展, 高亮, 张娟娟, 林拥军. 植物质体基因工程：新的优化策略及应用[J]. 遗传, 2015, 37(8): 777-792.

Fei Zhou, Shizhan Lu, Liang Gao, Juanjuan Zhang, Yongjun Lin. Plastid genome engineering: novel optimization strategies and applications[J]. HEREDITAS(Beijing), 2015, 37(8): 777-792.

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植物质体基因工程：新的优化策略及应用

Plastid genome engineering: novel optimization strategies and applications

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